PDF(Pubmed)
Abstract：
The present study evaluated the repercussions of magnetopriming on the root system architecture of soybean plants subjected to arsenic toxicity using synchrotron radiation source based micro-computed tomography (SR-µCT). This will be used evey where as abbreviation for the technique for three-dimensional imaging. Seeds of soybean were exposed to the static magnetic field (SMF) of strength (200 mT) for 1h prior to sowing. Magnetoprimed and non-primed seeds were grown for 1 month in a soil-sand mixture containing four different levels of sodium arsenate (0, 5, 10, and 50 mg As kg-1 soil). The results showed that arsenic adversely affects the root growth in non-primed plants by reducing their root length, root biomass, root hair, size and number of root nodules, where the damaging effect of As was observed maximum at higher concentrations (10 and 50 mg As kg-1 soil). However, a significant improvement in root morphology was detected in magnetoprimed plants where SMF pretreatment enhanced the root length, root biomass, pore diameter of cortical cells, root hair formation, lateral roots branching, and size of root nodules and girth of primary roots. Qualitative analysis of x-ray micro-CT images showed that arsenic toxicity damaged the epidermal and cortical layers of the root as well as reduced the pore diameter of the cortical cells. However, the diameter of cortical cells pores in magnetoprimed plants was observed higher as compared to plants emerged from non-primed seeds at all level of As toxicity. Thus, the study suggested that magnetopriming has the potential to attenuate the toxic effect of As and could be employed as a pre-sowing treatment to reduce the phytotoxic effects of metal ions in plants by improving root architecture and root tolerance index. This study is the very first exploration of the potential benefits of magnetopriming in mitigating the toxicity of metals (As) in plant roots utilizing the micro-CT technique.
摘要：
本研究使用基于同步加速器辐射源的微型计算机断层扫描（SR-µCT）评估了磁蚀对遭受砷毒性的大豆植物根系结构的影响。这将被用作三维成像技术的缩写。大豆的种子在播种前暴露于强度（200mT）的静磁场（SMF）1小时。在含有四种不同水平的砷酸钠（0、5、10和50mgAskg-1土壤）的土壤-沙子混合物中，将磁石和未引发的种子生长1个月。结果表明，砷通过减少其根长，对未引发植物的根生长产生不利影响，根系生物量,根毛,根瘤的大小和数量，其中在较高浓度（10和50mgAskg-1土壤）下观察到As的破坏作用最大。然而,在SMF预处理增强根长的磁纹植物中检测到根形态的显着改善，根系生物量,皮质细胞的孔径，根毛形成，侧根分枝，根瘤的大小和初根的周长。X线显微CT图像的定性分析表明，砷毒性破坏了根的表皮层和皮质层，并减小了皮质细胞的孔径。然而,在所有As毒性水平下，与未引发的种子中出现的植物相比，观察到磁结晶体植物中皮质细胞孔的直径更高。因此，该研究表明，磁三化具有减弱As的毒性作用的潜力，可以用作播种前处理，通过改善根系结构和根系耐性指数来降低植物中金属离子的植物毒性作用。这项研究是首次探索利用Micro-CT技术减轻植物根部金属（As）毒性的磁粉化潜在益处。
