Abstract：
The escalating use of nanodiamonds (NDs) has raised concerns about their ecotoxicological impact, prompting exploration of therapeutic interventions. This paper pioneers the examination of Vitamin B12-conjugated sericin (VB12-SER) as a potential therapeutic approach against ND-induced toxicity in darkling beetles (Blaps polychresta). The study analyzes mortality rates and organ-specific effects, covering the testis, ovary, and midgut, before and after treatments. Following exposure to 10 mg NDs/g body weight, within a subgroup of individuals termed ND2 with a mortality rate below 50%, two therapeutic treatments were administered, including pure sericin (SER) at 10 mg/mL and VB12-SER at 10.12 mg/mL. Consequently, five experimental groups (control, SER, ND2, ND2+SER, ND2+SER+VB12) were considered. Kaplan-Meier survival analysis was performed to assess the lifespan distribution of the insects in these groups over a 30-d period. Analyses revealed increased mortality and significant abnormalities induced by NDs within the examined organs, including cell death, DNA damage, enzyme dysregulation, antioxidant imbalances, protein depletion, lipid peroxidation, and morphological deformities. In contrast, the proposed treatments, especially (ND2+SER+VB12), demonstrated remarkable recovery, highlighting VB12-conjugated SER\'s potential in mitigating ND-triggered adverse effects. Molecular docking simulations affirmed binding stability and favorable interactions of the VB12-SER complex with target proteins. This research enhances understanding of NDs\' effects on B. polychresta, proposing it as an effective bioindicator, and introduces VB12-conjugated SER as a promising therapeutic strategy in nanotoxicological studies.
摘要：
纳米金刚石（ND）的使用不断升级，引起了人们对其生态毒理学影响的担忧，促使探索治疗干预措施。本文率先研究了维生素B12结合的丝胶蛋白（VB12-SER）作为一种潜在的治疗方法，以对抗ND引起的黑暗甲虫（Blapspolychresta）毒性。该研究分析了死亡率和器官特异性效应，覆盖睾丸，子房,和中肠，治疗前后。暴露于10mgNDs/g体重后，在死亡率低于50%的称为ND2的个体亚组中，进行了两种治疗，包括10mg/mL的纯丝胶(SER)和10.12mg/mL的VB12-SER。因此,五个实验组(对照组，SER,ND2,ND2+SER,考虑ND2+SER+VB12)。进行Kaplan-Meier存活分析以评估这些组中昆虫在30天期间的寿命分布。分析显示，死亡率增加和被检查器官内的ND引起的显著异常，包括细胞死亡,DNA损伤,酶失调，抗氧化剂失衡，蛋白质消耗，脂质过氧化，和形态畸形。相比之下,拟议的治疗方法，尤其是（ND2+SER+VB12），表现出显著的恢复，突出显示VB12-共轭SER在减轻ND触发的不良反应方面的潜力。分子对接模拟证实了VB12-SER复合物与靶蛋白的结合稳定性和有利的相互作用。这项研究增强了对NDs对多chresta芽孢杆菌的影响的理解，提议它作为一种有效的生物指示剂，并介绍了VB12缀合的SER作为纳米毒理学研究中一种有前途的治疗策略。
