Abstract：
Water buffalo (Bubalus bubalis) farming is increasing in many regions of the world due to the species\' ability to thrive in environments where bovine cattle would struggle. Despite water buffaloes being known for their resistance to diseases, there is a lack of data about the diversity of the microbiome of the species. In this study, we examined the virome diversity in palatine tonsils collected from animals from the island of Marajó, northern Pará state, Brazil, which harbors the largest bubaline flock in the country. Tonsil fragments from 60 clinically healthy bubalines were randomly selected from a sample of 293 animals. The samples were purified, extracted, and randomly amplified with phi29 DNA polymerase. After amplification, the products were purified and sequenced. Circular DNA viruses were predominant in the tonsils\' virome. Sequences of genome segments representative of members of the genera Alphapolyomavirus (including a previously unreported bubaline polyomavirus genome) and Gemycircularvirus were identified, along with other not yet classified circular virus genomes. As the animals were clinically healthy at the time of sampling, such viruses likely constitute part of the normal tonsillar virome of water buffaloes inhabiting the Ilha do Marajó biome.
摘要：
水牛（Bubalusbubalis）养殖在世界许多地区都在增加，因为该物种能够在牛牛挣扎的环境中茁壮成长。尽管水牛以抵抗疾病而闻名，缺乏有关该物种微生物组多样性的数据。在这项研究中,我们检查了从马拉霍岛的动物身上采集的腭扁桃体中的病毒多样性，帕拉州北部,巴西,这是全国最大的布巴林羊群。从293只动物的样品中随机选择来自60个临床上健康的布巴林的扁桃体片段。样品经过纯化,提取,用phi29DNA聚合酶随机扩增。扩增后,对产物进行纯化和测序。环状DNA病毒在扁桃体病毒中占主导地位。鉴定了代表α多瘤病毒属成员的基因组片段序列（包括以前未报道的布巴林多瘤病毒基因组）和双环病毒，以及其他尚未分类的环状病毒基因组。由于取样时动物临床健康，此类病毒可能构成居住在IlhadoMarajo生物群落中的水牛的正常扁桃体病毒的一部分。
