Abstract：
Liamocins are molecules with a polyol lipid structure produced by rare strains of Aureobasidium pullulans. In recent years, liamocins have attracted attention due to their antibacterial, anticancer and surface-active properties, and promising potential applications have been identified in the food, agriculture, medical and pharmaceutical industries. This study is the first to investigate the effects of different carbon and nitrogen sources on the growth and liamocin production kinetics of A. pullulans NBRC 100716 strain. This strain was selected among six different A. pullulans strains whose liamocin productions were tested by us for the first time. In fermentations carried out in shaking water baths, the carbon source that most supported the liamocin production of this strain was fructose, and the nitrogen source was peptone-yeast extract combination. In the medium containing fructose and the peptone-yeast extract mixture, A. pullulans NBRC 100716 produced 4.26 g liamocin L-1. The specific liamocin production rate (qp) of the strain in this medium was 0.0090 g liamocin/g mo.h. This study is also the first to produce liamocin with a fructophilic A. pullulans strain. Present findings in this research also demonstrated the excellent biosurfactant capacity of the liamocin produced by this strain. The obtained liamocin reduced the water surface tension to a degree that can compete with synthetic surfactants. Furthermore, this is the first report to reveal that the fatty acid profile of liamocin obtained from A. pullulans NBRC 100716 contains an appreciable amount of unsaturated fatty acids and is similar to the composition of vegetable oil.
摘要：
Liamocin是具有由稀有的普鲁兰梭菌菌株产生的多元醇脂质结构的分子。近年来,由于它们的抗菌作用，Liamocin引起了人们的注意，抗癌和表面活性特性，并在食品中确定了有希望的潜在应用，农业,医疗和制药行业。本研究首次研究了不同碳源和氮源对支链淀粉NBRC100716菌株生长和产生激光霉素动力学的影响。该菌株是在六种不同的普鲁兰杆菌菌株中选择的，我们首次测试了它们的liamocin产生。在摇动水浴中进行的发酵中，最支持该菌株产生可可碱的碳源是果糖，氮源为蛋白胨-酵母提取物组合。在含有果糖和蛋白胨-酵母提取物混合物的培养基中，A.普鲁兰NBRC100716产生了4.26克的维生素L-1。该菌株在该培养基中的特定的金属霉素生产速率(qp)为0.0090g金属霉素/gmo.h。该研究也是第一个用嗜果糖支链淀粉杆菌菌株生产金属霉素的研究。本研究的发现还证明了该菌株产生的liamocin具有出色的生物表面活性剂能力。所获得的Liamocin将水表面张力降低到可以与合成表面活性剂竞争的程度。此外，这是第一份报告，揭示从A.pullulansNBRC100716获得的棕榈碱的脂肪酸谱含有相当数量的不饱和脂肪酸，类似于植物油的组成。
