PDF(Pubmed)
Abstract：
Conversion of heterotrophic organisms into partially or completely autotrophic organisms is primarily accomplished by extensive metabolic engineering and laboratory evolution efforts that channel CO2 into central carbon metabolism. Here, we develop a directed endosymbiosis approach to introduce carbon assimilation in budding yeasts. Particularly, we engineer carbon assimilating and sugar-secreting photosynthetic cyanobacterial endosymbionts within the yeast cells, which results in the generation of yeast/cyanobacteria chimeras that propagate under photosynthetic conditions in the presence of CO2 and in the absence of feedstock carbon sources like glucose or glycerol. We demonstrate that the yeast/cyanobacteria chimera can be engineered to biosynthesize natural products under the photosynthetic conditions. Additionally, we expand our directed endosymbiosis approach to standard laboratory strains of yeasts, which transforms them into photosynthetic yeast/cyanobacteria chimeras. We anticipate that our studies will have significant implications for sustainable biotechnology, synthetic biology, and experimentally studying the evolutionary adaptation of an additional organelle in yeast.
摘要：
异养生物向部分或完全自养生物的转化主要是通过广泛的代谢工程和实验室进化努力来实现的，这些努力将CO2引导到中心碳代谢中。这里,我们开发了一种定向内共生方法来在出芽酵母中引入碳同化。特别是,我们在酵母细胞内设计碳同化和分泌糖的光合蓝细菌内共生体，这导致产生酵母/蓝细菌嵌合体,其在存在CO2和不存在原料碳源如葡萄糖或甘油的情况下在光合条件下繁殖。我们证明了酵母/蓝细菌嵌合体可以在光合条件下进行工程改造以生物合成天然产物。此外,我们将定向内共生方法扩展到标准的实验室酵母菌株，将它们转化为光合酵母/蓝细菌嵌合体。我们预计我们的研究将对可持续生物技术产生重大影响，合成生物学,并通过实验研究酵母中另一个细胞器的进化适应。
