OBJECTIVE: To evaluate uTIMP-2, uIGFBP7, fractional excretion of NGAL (FeNGAL), and urinary to serum NGAL ratio (u/sNGAL) in healthy dogs, dogs with AKI, dogs with chronic kidney disease (CKD), and critically ill (CI) dogs.
METHODS: Forty-two client-owned dogs (healthy, n = 10; AKI, n = 11; CKD, n = 11; CI, n = 10).
METHODS: Prospective, observational study. After assessment of routine renal biomarkers, stress (uTIMP-2, uIGFBP7) and damage (NGAL) biomarkers were measured, using ELISA kits, and normalized to urinary creatinine (uCr).
RESULTS: Normalized uTIMP-2 and [uTIMP-2] × [uIGFBP7]/uCr were significantly higher in the AKI group (median 151.9 [range, 2.2-534.2] and 62.9 [1.1-266.8] pg/mL respectively), compared to healthy dogs (0.3 [0.2-74.7]; P < .001 and 0.16 [0.1-58.1] pg/mL; P < .001), dogs with CKD (0.7 [0.3-742.5]; P = .04 and 0.37 [0.2-180.1] pg/mL; P = .03) and CI dogs (1.9 [0.2-37.0]; P = .03 and 0.8 [0.1-16.1] pg/mL; P = .02). Fractional excretion of NGAL was significantly higher in dogs with AKI (54.17 [7.93-155.32] %), than in healthy (0.03 [0.01-0.21] %; P < .001) and CI dogs (3.05 [0.05-28.86] %; P = .02).
CONCLUSIONS: Normalized uTIMP-2, [uTIMP-2] × [uIGFBP7]/uCr, and FeNGAL can be valuable renal biomarkers for early diagnosis of AKI in dogs.
目的:评估uTIMP-2,uIGFBP7,NGAL的排泄分数(FeNGAL),和健康狗的尿与血清NGAL比率(u/sNGAL),有AKI的狗,患有慢性肾病(CKD)的狗,和重症(CI)狗。
方法:42只客户拥有的狗(健康,n=10;AKI,n=11;CKD,n=11;CI,n=10)。
方法:前瞻性,观察性研究。在评估常规肾脏生物标志物后,测量应激(uTIMP-2,uIGFBP7)和损伤(NGAL)生物标志物,使用ELISA试剂盒,并对尿肌酐(uCr)进行归一化。
结果:归一化uTIMP-2和[uTIMP-2]×[uIGFBP7]/uCr在AKI组中明显更高(中位数151.9[范围,2.2-534.2]和62.9[1.1-266.8]pg/mL),与健康狗相比(0.3[0.2-74.7];P<.001和0.16[0.1-58.1]pg/mL;P<.001),CKD犬(0.7[0.3-742.5];P=.04和0.37[0.2-180.1]pg/mL;P=.03)和CI犬(1.9[0.2-37.0];P=.03和0.8[0.1-16.1]pg/mL;P=.02)。NGAL的排泄分数在患有AKI的狗中显著更高(54.17[7.93-155.32]%),健康犬(0.03[0.01-0.21]%;P<.001)和CI犬(3.05[0.05-28.86]%;P=.02)。
结论:归一化uTIMP-2,[uTIMP-2]×[uIGFBP7]/uCr,FeNGAL可以作为早期诊断犬AKI的肾脏生物标志物。