Abstract：
Gellan gum (GG) is a soft, tractable, and natural polysaccharide substrate used for cell incubation. In this study, we examined the effects of GG on porcine oocyte maturation. Cumulus cells and oocyte complexes (COCs) were collected from slaughterhouse-derived porcine ovaries and cultured on plastic plates containing 0.05% or 0.1% GG gels. The 0.1% GG gel improved the maturation rate and quality of blastocysts, as determined by the total cell number and the rate of abnormally condensed nuclei. GG gels have antioxidant abilities and oocytes cultured on GG gels (0.05% and 0.1%) have reduced reactive oxygen species (ROS) content. Furthermore, GG gels (0.05% and 0.1%) increased F-actin formation, whereas treatment of oocytes with H2O2 reduced F-actin levels. GG gels increased the ATP content in oocytes but did not affect the mitochondrial DNA copy number or mitochondrial membrane potential. In addition, the medium cultured on 0.05% GG increased the glucose consumption of COCs. In conclusion, GG gel reduced ROS content, increased energy content, and improved subsequent embryonic development in pigs.
摘要：
结冷胶（GG）是一种软，易处理，和用于细胞培养的天然多糖底物。在这项研究中,我们研究了GG对猪卵母细胞成熟的影响。从屠宰场衍生的猪卵巢中收集卵丘细胞和卵母细胞复合物（COC），并在含有0.05％或0.1％GG凝胶的塑料板上培养。0.1%GG凝胶提高了胚泡的成熟率和质量,由总细胞数和异常凝聚核的比率决定。GG凝胶具有抗氧化能力，在GG凝胶上培养的卵母细胞（0.05％和0.1％）具有降低的活性氧（ROS）含量。此外，GG凝胶（0.05％和0.1％）增加了F-肌动蛋白的形成，而用H2O2处理卵母细胞可降低F-肌动蛋白水平。GG凝胶增加了卵母细胞中的ATP含量，但不影响线粒体DNA拷贝数或线粒体膜电位。此外,在0.05％GG上培养的培养基增加了COCs的葡萄糖消耗。总之,GG凝胶降低ROS含量，能量含量增加，并改善了猪随后的胚胎发育。
