METHODS: We obtained a total of 16 Gb and 51 Gb PacBio HiFi data for O. transversa and C. friedae, respectively, which were assembled into the draft genomes of 339 Mb for O. transversa and 233 Mb for C. friedae. Genome assessments by multiple metrics showed good completeness and high accuracy of the two assemblies. Gene annotations assisted by RNA-seq data yielded a comparable number of protein-coding genes in the two genomes (10,892 for O. transversa and 11,296 for C. friedae), while repeat annotations revealed a remarkable difference of repeat content between these two ant species (149.4 Mb for O. transversa versus 49.7 Mb for C. friedae). Besides, complete mitochondrial genomes for the two species were assembled and annotated.
方法:我们获得了O.transversa和C.friedae的16Gb和51GbPacBioHiFi数据,分别,将其组装到O.transversa的339Mb和C.friedae的233Mb的草案基因组中。通过多个度量进行的基因组评估显示了两个组装的良好完整性和高精度。RNA-seq数据辅助的基因注释在两个基因组中产生了相当数量的蛋白质编码基因(O.transversa为10,892,C.friedae为11,296),而重复注释显示,这两种蚂蚁之间的重复含量存在显着差异(O.transversa为149.4Mb,而Friedae为49.7Mb)。此外,组装并注释了这两个物种的完整线粒体基因组。