PDF(Pubmed)
Abstract：
OBJECTIVE: Ants are ecologically dominant insects in most terrestrial ecosystems, with more than 14,000 extant species in about 340 genera recorded to date. However, genomic resources are still scarce for most species, especially for species endemic in East or Southeast Asia, limiting the study of phylogeny, speciation and adaptation of this evolutionarily successful animal lineage. Here, we assemble and annotate the genomes of Odontoponera transversa and Camponotus friedae, two ant species with a natural distribution in China, to facilitate future study of ant evolution.
METHODS: We obtained a total of 16 Gb and 51 Gb PacBio HiFi data for O. transversa and C. friedae, respectively, which were assembled into the draft genomes of 339 Mb for O. transversa and 233 Mb for C. friedae. Genome assessments by multiple metrics showed good completeness and high accuracy of the two assemblies. Gene annotations assisted by RNA-seq data yielded a comparable number of protein-coding genes in the two genomes (10,892 for O. transversa and 11,296 for C. friedae), while repeat annotations revealed a remarkable difference of repeat content between these two ant species (149.4 Mb for O. transversa versus 49.7 Mb for C. friedae). Besides, complete mitochondrial genomes for the two species were assembled and annotated.
METHODS: We obtained a total of 16 Gb and 51 Gb PacBio HiFi data for O. transversa and C. friedae, respectively, which were assembled into the draft genomes of 339 Mb for O. transversa and 233 Mb for C. friedae. Genome assessments by multiple metrics showed good completeness and high accuracy of the two assemblies. Gene annotations assisted by RNA-seq data yielded a comparable number of protein-coding genes in the two genomes (10,892 for O. transversa and 11,296 for C. friedae), while repeat annotations revealed a remarkable difference of repeat content between these two ant species (149.4 Mb for O. transversa versus 49.7 Mb for C. friedae). Besides, complete mitochondrial genomes for the two species were assembled and annotated.
摘要：
目的：在大多数陆地生态系统中，蚂蚁是生态优势昆虫，迄今为止，已有约340属的14,000多个现存物种。然而,对于大多数物种来说，基因组资源仍然稀缺，尤其是东亚或东南亚特有的物种，限制了系统发育的研究，这种进化上成功的动物谱系的物种形成和适应。这里,我们组装并注释了Odontoponeratransversa和Camponotusfriedae的基因组，两种在中国自然分布的蚂蚁，以促进未来对蚂蚁进化的研究。
方法：我们获得了O.transversa和C.friedae的16Gb和51GbPacBioHiFi数据，分别,将其组装到O.transversa的339Mb和C.friedae的233Mb的草案基因组中。通过多个度量进行的基因组评估显示了两个组装的良好完整性和高精度。RNA-seq数据辅助的基因注释在两个基因组中产生了相当数量的蛋白质编码基因（O.transversa为10,892，C.friedae为11,296），而重复注释显示，这两种蚂蚁之间的重复含量存在显着差异（O.transversa为149.4Mb，而Friedae为49.7Mb）。此外,组装并注释了这两个物种的完整线粒体基因组。
方法：我们获得了O.transversa和C.friedae的16Gb和51GbPacBioHiFi数据，分别,将其组装到O.transversa的339Mb和C.friedae的233Mb的草案基因组中。通过多个度量进行的基因组评估显示了两个组装的良好完整性和高精度。RNA-seq数据辅助的基因注释在两个基因组中产生了相当数量的蛋白质编码基因（O.transversa为10,892，C.friedae为11,296），而重复注释显示，这两种蚂蚁之间的重复含量存在显着差异（O.transversa为149.4Mb，而Friedae为49.7Mb）。此外,组装并注释了这两个物种的完整线粒体基因组。
