PDF(Pubmed)
Abstract：
Echinococcus spp. is an emerging zoonotic parasite of high concern. In Canada, an increase in the number of human and animal cases diagnosed has been reported, but information regarding the parasite\'s distribution in wildlife reservoir remains limited. A cross-sectional study was conducted to estimate the prevalence of wild canids infected with Echinococcus spp. and Echinococcus multilocularis in areas surrounding populated zones in Québec (Canada); to investigate the presence of areas at higher risk of infection; to evaluate potential risk factors of the infection; and as a secondary objective, to compare coproscopy and RT-PCR diagnostic tests for Taenia spp. and Echinococcus identification. From October 2020 to March 2021, fecal samples were collected from 423 coyotes (Canis latrans) and 284 red foxes (Vulpes vulpes) trapped in 12 administrative regions. Real-time PCR for molecular detection of genus Echinococcus spp. and species-specific Echinococcus multilocularis were performed. A total of 38 positive cases of Echinococcus spp., of which 25 were identified as E. multilocularis, were detected. Two high-risk areas of infection were identified. The prevalence of Echinococcus spp. was 22.7% (95% CI 11.5-37.8%) in the Montérégie centered high-risk area, 26.5% (95% CI 12.9-44.4%) in the Bas-St-Laurent high-risk area, and 3.0% (95%CI 1.8-4.7%) outside those areas. For E. multilocularis, a prevalence of 20.5% (95% CI 9.8-35.3%) was estimated in the high-risk area centered in Montérégie compared to 2.4% (95% CI 1.4-3.9%) outside. Logistic regression did not show any association of infection status with species, sex, or geolocation of capture (p > 0.05). This study shows the circulation of Echinococcus in a wildlife cycle in 9/12 administrative regions of Québec.
摘要：
棘球蚴属。是一种备受关注的新兴人畜共患寄生虫。在加拿大,据报道，诊断的人类和动物病例数量有所增加，但有关寄生虫在野生动物水库中分布的信息仍然有限。进行了一项横断面研究，以估计感染棘球蚴的野生犬科动物的患病率。魁北克（加拿大）人口稠密地区的多房棘球蚴；调查感染风险较高的地区的存在；评估感染的潜在危险因素；作为次要目标，比较共镜检查和RT-PCR对带虫属的诊断试验。和棘球蚴的鉴定。从2020年10月到2021年3月，收集了被困在12个行政区的423只土狼（Canislatrans）和284只红狐狸（Vulpesvulpes）的粪便样本。实时荧光定量PCR技术用于棘球蚴属的分子检测。并进行了特定物种的多房棘球蚴。共38例棘球蚴阳性。,其中25个被鉴定为多房性大肠杆菌，被检测到。确定了两个高危感染区域。棘球蚴的患病率。在以蒙特雷吉为中心的高风险地区为22.7%(95%CI11.5-37.8%)，Bas-St-Laurent高风险地区的26.5％（95％CI12.9-44.4％），在这些地区之外，还有3.0%（95CI1.8-4.7%）。对于多房性大肠杆菌，在以蒙特雷吉为中心的高危地区,患病率估计为20.5%(95%CI9.8-35.3%),而在其他地区为2.4%(95%CI1.4-3.9%).Logistic回归没有显示感染状态与物种的任何关联，性别,或捕获的地理位置(p>0.05)。这项研究显示了魁北克9/12行政区野生动物轮回中棘球蚴的循环。
