PDF(Pubmed)
Abstract：
Artemisia argyi, a perennial herb of the genus Artemisia in the family Asteraceae, holds significant importance in Chinese traditional medicine, referred to as \"Aicao\". Here, we report a high-quality reference genome of Artemisia argyi L. cv. beiai, with a genome size up to 4.15 Gb and a contig N50 of 508.96 Kb, produced with third-generation Nanopore sequencing technology. We predicted 147,248 protein-coding genes, with approximately 68.86% of the assembled sequences comprising repetitive elements, primarily long terminal repeat retrotransposons(LTRs). Comparative genomics analysis shows that A. argyi has the highest number of specific gene families with 5121, and much more families with four or more members than the other 6 plant species, which is consistent with its more expanded gene families and fewer contracted gene families. Furthermore, through transcriptome sequencing of A. argyi in response to exogenous MeJA treatment, we have elucidated acquired regulatory insights into MeJA\'s impact on the phenylpropanoid, flavonoid, and terpenoid biosynthesis pathways of A. argyi. The whole-genome information obtained in this study serves as a valuable resource for delving deeper into the cultivation and molecular breeding of A. argyi. Moreover, it holds promise for enhancing genome assemblies across other members of the Asteraceae family. The identification of key genes establishes a solid groundwork for developing new varieties of Artemisia with elevated concentrations of active compounds.
摘要：
Artemisiaargyi,菊科蒿属的多年生草本植物，在中国传统医学中具有重要意义，称为“Aicao”。这里,我们报告了艾蒿的高质量参考基因组。贝艾，基因组大小高达4.15Gb，重叠群N50为508.96Kb，用第三代纳米孔测序技术生产。我们预测了147,248个蛋白质编码基因，大约68.86%的组装序列包含重复元素，主要是长末端重复反转录转座子（LTR）。比较基因组学分析表明，阿吉的特定基因家族数量最多，为5121个，并且具有四个或四个以上成员的家族比其他6个植物物种多得多，这与它更扩展的基因家族和更少的签约基因家族是一致的。此外，通过对A.argyi响应于外源MeJA处理的转录组测序，我们已经阐明了对MeJA对苯丙素的影响的获得的监管见解，类黄酮，和萜类生物合成途径。在这项研究中获得的全基因组信息为深入研究A.argyi的栽培和分子育种提供了宝贵的资源。此外，它有望增强菊科其他成员的基因组组装。关键基因的鉴定为开发具有高浓度活性化合物的蒿新品种奠定了坚实的基础。
