PDF(Pubmed)
Abstract：
UNASSIGNED: Pulmonary neutrophilia is a hallmark of numerous airway diseases including Chronic Obstructive Pulmonary Disease (COPD), Neutrophilic asthma, Acute Lung Injury (ALI), Acute Respiratory Distress Syndrome (ARDS) and COVID-19. The aim of the current study was to investigate the effect of dietary interventions on lung health in context of pulmonary neutrophilia.
UNASSIGNED: Male BALB/cByJ mice received 7 intra-nasal doses of either a vehicle or lipopolysaccharides (LPS). To study the effect of nutritional interventions they received 16 intra-gastric doses of either a vehicle (PBS) or the following supplements (1) probiotic Bifidobacterium breve (B. breve) M16-V; (2) a prebiotic fiber mixture of short-chain galacto-oligosaccharides, long-chain fructo-oligosaccharides, and low-viscosity pectin in a 9:1:2 ratio (scGOS/lcFOS/lvPectin); and (3) A synbiotic combination B. breve M16-V and scGOS/lcFOS/lvPectin. Parameters for lung health included lung function, lung morphology and lung inflammation. Parameters for systemic immunomodulation included levels of fecal short chain fatty acids and regulatory T cells.
UNASSIGNED: The synbiotic supplement protected against the LPS induced decline in lung function (35% improved lung resistance at baseline p = 0.0002 and 25% at peak challenge, p = 0.0002), provided a significant relief from pulmonary neutrophilia (40.7% less neutrophils, p < 0.01) and improved the pulmonary neutrophil-to-lymphocyte ratio (NLR) by 55.3% (p = 0.0033). Supplements did not impact lung morphology in this specific experiment. LPS applied to the upper airways induced less fecal SCFAs production compared to mice that received PBS. The production of acetic acid between day -5 and day 16 was increased in all unchallenged mice (PBS-PBS p = 0.0003; PBS-Pro p < 0.0001; PBS-Pre, p = 0.0045; PBS-Syn, p = 0.0005) which upon LPS challenge was only observed in mice that received the synbiotic mixture of B. breve M16-V and GOS:FOS:lvPectin (p = 0.0003). A moderate correlation was found for butyric acid and lung function parameters and a weak correlation was found between acetic acid, butyric acid and propionic acid concentrations and NLR.
UNASSIGNED: This study suggests bidirectional gut lung cross-talk in a mouse model for pulmonary neutrophilia. Neutrophilic lung inflammation coexisted with attenuated levels of fecal SCFA. The beneficial effects of the synbiotic mixture of B. breve M16-V and GOS:FOS:lvPectin on lung health associated with enhanced levels of SCFAs.
UNASSIGNED: Male BALB/cByJ mice received 7 intra-nasal doses of either a vehicle or lipopolysaccharides (LPS). To study the effect of nutritional interventions they received 16 intra-gastric doses of either a vehicle (PBS) or the following supplements (1) probiotic Bifidobacterium breve (B. breve) M16-V; (2) a prebiotic fiber mixture of short-chain galacto-oligosaccharides, long-chain fructo-oligosaccharides, and low-viscosity pectin in a 9:1:2 ratio (scGOS/lcFOS/lvPectin); and (3) A synbiotic combination B. breve M16-V and scGOS/lcFOS/lvPectin. Parameters for lung health included lung function, lung morphology and lung inflammation. Parameters for systemic immunomodulation included levels of fecal short chain fatty acids and regulatory T cells.
UNASSIGNED: The synbiotic supplement protected against the LPS induced decline in lung function (35% improved lung resistance at baseline p = 0.0002 and 25% at peak challenge, p = 0.0002), provided a significant relief from pulmonary neutrophilia (40.7% less neutrophils, p < 0.01) and improved the pulmonary neutrophil-to-lymphocyte ratio (NLR) by 55.3% (p = 0.0033). Supplements did not impact lung morphology in this specific experiment. LPS applied to the upper airways induced less fecal SCFAs production compared to mice that received PBS. The production of acetic acid between day -5 and day 16 was increased in all unchallenged mice (PBS-PBS p = 0.0003; PBS-Pro p < 0.0001; PBS-Pre, p = 0.0045; PBS-Syn, p = 0.0005) which upon LPS challenge was only observed in mice that received the synbiotic mixture of B. breve M16-V and GOS:FOS:lvPectin (p = 0.0003). A moderate correlation was found for butyric acid and lung function parameters and a weak correlation was found between acetic acid, butyric acid and propionic acid concentrations and NLR.
UNASSIGNED: This study suggests bidirectional gut lung cross-talk in a mouse model for pulmonary neutrophilia. Neutrophilic lung inflammation coexisted with attenuated levels of fecal SCFA. The beneficial effects of the synbiotic mixture of B. breve M16-V and GOS:FOS:lvPectin on lung health associated with enhanced levels of SCFAs.
摘要：
■肺中性粒细胞增多症是许多气道疾病的标志，包括慢性阻塞性肺疾病（COPD），中性粒细胞性哮喘,急性肺损伤(ALI),急性呼吸窘迫综合征（ARDS）和COVID-19。本研究的目的是调查饮食干预对肺中性粒细胞增多的影响。
■雄性BALB/cByJ小鼠接受7个鼻内剂量的媒介物或脂多糖(LPS)。为了研究营养干预的效果，他们接受了16剂胃内剂量的媒介物（PBS）或以下补充剂（1）益生菌短双歧杆菌（B.breve）M16-V；（2）短链半乳寡糖的益生元纤维混合物，长链低聚果糖，和9:1:2比例的低粘度果胶(scGOS/lcFOS/lvPectin);和(3)合生元组合短芽孢杆菌M16-V和scGOS/lcFOS/lvPectin。肺健康参数包括肺功能，肺形态和肺部炎症。全身免疫调节的参数包括粪便短链脂肪酸和调节性T细胞的水平。
■合生元补充剂可防止LPS诱导的肺功能下降（基线p=0.0002时，肺阻力提高了35％，峰值时提高了25％，p=0.0002),提供了肺嗜中性粒细胞的显着缓解（减少了40.7％的嗜中性粒细胞，p<0.01），并将肺中性粒细胞与淋巴细胞的比率（NLR）提高了55.3％（p=0.0033）。在该特定实验中,补充剂不影响肺形态。与接受PBS的小鼠相比,应用于上气道的LPS诱导较少的粪便SCFA产生。在所有未攻击的小鼠中，在第-5天和第16天之间乙酸的产生增加（PBS-PBSp=0.0003；PBS-Prop<0.0001；PBS-Pre，p=0.0045;PBS-Syn,p=0.0005），仅在接受短双歧杆菌M16-V和GOS：FOS：lvPectin合生元混合物的小鼠中观察到LPS攻击（p=0.0003）。发现丁酸和肺功能参数之间存在中度相关性，发现乙酸之间存在弱相关性，丁酸和丙酸浓度和NLR。
■本研究提示了肺嗜中性粒细胞增多症的小鼠模型中的双向肠道肺串扰。中性粒细胞性肺部炎症与粪便SCFA水平减弱共存。短双歧杆菌M16-V和GOS:FOS:lvPectin的合生元混合物对与SCFA水平增强相关的肺健康的有益效果。
■雄性BALB/cByJ小鼠接受7个鼻内剂量的媒介物或脂多糖(LPS)。为了研究营养干预的效果，他们接受了16剂胃内剂量的媒介物（PBS）或以下补充剂（1）益生菌短双歧杆菌（B.breve）M16-V；（2）短链半乳寡糖的益生元纤维混合物，长链低聚果糖，和9:1:2比例的低粘度果胶(scGOS/lcFOS/lvPectin);和(3)合生元组合短芽孢杆菌M16-V和scGOS/lcFOS/lvPectin。肺健康参数包括肺功能，肺形态和肺部炎症。全身免疫调节的参数包括粪便短链脂肪酸和调节性T细胞的水平。
■合生元补充剂可防止LPS诱导的肺功能下降（基线p=0.0002时，肺阻力提高了35％，峰值时提高了25％，p=0.0002),提供了肺嗜中性粒细胞的显着缓解（减少了40.7％的嗜中性粒细胞，p<0.01），并将肺中性粒细胞与淋巴细胞的比率（NLR）提高了55.3％（p=0.0033）。在该特定实验中,补充剂不影响肺形态。与接受PBS的小鼠相比,应用于上气道的LPS诱导较少的粪便SCFA产生。在所有未攻击的小鼠中，在第-5天和第16天之间乙酸的产生增加（PBS-PBSp=0.0003；PBS-Prop<0.0001；PBS-Pre，p=0.0045;PBS-Syn,p=0.0005），仅在接受短双歧杆菌M16-V和GOS：FOS：lvPectin合生元混合物的小鼠中观察到LPS攻击（p=0.0003）。发现丁酸和肺功能参数之间存在中度相关性，发现乙酸之间存在弱相关性，丁酸和丙酸浓度和NLR。
■本研究提示了肺嗜中性粒细胞增多症的小鼠模型中的双向肠道肺串扰。中性粒细胞性肺部炎症与粪便SCFA水平减弱共存。短双歧杆菌M16-V和GOS:FOS:lvPectin的合生元混合物对与SCFA水平增强相关的肺健康的有益效果。
