PDF(Pubmed)
Abstract：
Acute hepatopancreatic necrosis (AHPND) is a severe bacterial disease affecting farmed shrimp. Although various pathogenic bacteria associated with AHPND-affected shrimp have been described, little is known about the bacterial signatures in the stomachs and intestines when the disease occurs naturally. In this study, we characterized the microbiome of P. vannamei by high-throughput sequencing (HTS). Shrimp samples were collected from a commercial farm and divided into two groups: healthy and affected by AHPND, confirmed by PCR. Stomach and intestine samples were subjected to microbiome analysis targeting the V3-V4 region of the 16S rRNA gene. PERMANOVA analysis revealed a significant disparity in the bacterial diversity between the stomach and intestine microbiomes of these two health conditions. Our results suggest that the significant abundance of Vibrio brasiliensis and V. sinaloensis in the intestines of affected shrimp plays a role in AHPND infection. This imbalance could be mitigated by the presence of Pseudoalteromonas, Gilvimarinus, and other members of the phylum Pseudomonadota such as Cellvibrionaceae, Psychromonadaceae, and Halieaceae, which showed significant abundance in healthy intestines. This study highlights the significance of the microbial community in the differentiation of specific microbial signatures in different organs of P. vannamei. These findings offer a deeper understanding of the intricate dynamics within the shrimp microbiome under these conditions, enriching our view of AHPND progression and paving the way toward future identification of probiotics tailored for more efficient management of this disease.
摘要：
急性肝胰腺坏死（AHPND）是一种严重的细菌性疾病，影响养殖虾。尽管已经描述了与受AHPND影响的虾相关的各种致病菌，当疾病自然发生时，人们对胃和肠道中的细菌特征知之甚少。在这项研究中,我们通过高通量测序（HTS）表征了南美白对虾的微生物组。从一个商业农场收集虾样本，分为两组：健康和受AHPND影响，通过PCR确认。对胃和肠样品进行靶向16SrRNA基因的V3-V4区的微生物组分析。PERMANOVA分析显示，这两种健康状况的胃和肠微生物组之间的细菌多样性存在显着差异。我们的结果表明，受影响的虾的肠道中大量的巴西弧菌和中华弧菌在AHPND感染中起作用。假单胞菌的存在可以缓解这种不平衡，Gilvimarinus,以及假单胞菌门的其他成员，如虫子科，嗜冷科,和卤科，在健康的肠道中显示出明显的丰度。这项研究强调了微生物群落在凡纳滨对虾不同器官中特定微生物特征分化中的重要性。这些发现为在这些条件下虾微生物组的复杂动态提供了更深入的理解，丰富了我们对AHPND进展的看法，并为将来确定为更有效地管理该疾病而量身定制的益生菌铺平了道路。
