PDF(Pubmed)
Abstract：
UNASSIGNED: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial and community acquired infections. Nanoparticles are considered as proper tools to overcome the therapeutic problem of antimicrobial-resistant infections because of the drug concentration increment at the desired location and protection from enzymatic degradation. The goal of this study was to evaluate the effect of the antibacterial and antibiofilm activities of zingerone and niosome containing zingerone against pre-formed biofilm of MRSA isolates.
UNASSIGNED: 62 MRSA isolates cultured from patients with diabetic ulcers were investigated. Niosomes were synthesized and characterized by X-ray diffraction, zeta potential and scanning electron microscopy (SEM). The size of niosomal particles measured by SEM and zetasizer.
UNASSIGNED: The surface charge of prepared niosomes was about -37 mV. The effect of the zingerone and noisome containing zingerone was evaluated against biofilms of MRSA isolates. Also, the antibiofilm activity of prepared niosomes on gene expression of MRSA biofilms was evaluated using Real Time PCR. Our results demonstrated that the niosome containing zingerone had a diameter of 196.1 nm and a -37.3-mV zeta potential. Zingerone removed one and three-day old biofilms of MRSA at the concentration of 1000 μg/ml, while the zingerone-laoded niosomes removed 1, 3- and 5-days old biofilms at the concentration of 250 μg/ml, 250 μg/ml, and 500 μg/ml.
UNASSIGNED: The results indicated that niosome containing zingerone eliminated MRSA and its biofilms faster compared with free zingerone and it suggested that zingerone-encapsulated niosomes could be considered as a promising treatment against MRSA and its biofilms.
UNASSIGNED: 62 MRSA isolates cultured from patients with diabetic ulcers were investigated. Niosomes were synthesized and characterized by X-ray diffraction, zeta potential and scanning electron microscopy (SEM). The size of niosomal particles measured by SEM and zetasizer.
UNASSIGNED: The surface charge of prepared niosomes was about -37 mV. The effect of the zingerone and noisome containing zingerone was evaluated against biofilms of MRSA isolates. Also, the antibiofilm activity of prepared niosomes on gene expression of MRSA biofilms was evaluated using Real Time PCR. Our results demonstrated that the niosome containing zingerone had a diameter of 196.1 nm and a -37.3-mV zeta potential. Zingerone removed one and three-day old biofilms of MRSA at the concentration of 1000 μg/ml, while the zingerone-laoded niosomes removed 1, 3- and 5-days old biofilms at the concentration of 250 μg/ml, 250 μg/ml, and 500 μg/ml.
UNASSIGNED: The results indicated that niosome containing zingerone eliminated MRSA and its biofilms faster compared with free zingerone and it suggested that zingerone-encapsulated niosomes could be considered as a promising treatment against MRSA and its biofilms.
摘要：
耐甲氧西林金黄色葡萄球菌（MRSA）是医院和社区获得性感染的主要原因。纳米颗粒被认为是克服抗微生物耐药性感染的治疗问题的适当工具，因为药物浓度在所需位置增加并保护免受酶降解。这项研究的目的是评估姜酮和含姜酮的niosome的抗菌和抗生物膜活性对MRSA分离株预先形成的生物膜的影响。
■研究了从糖尿病溃疡患者中培养的62株MRSA分离物。合成了Niosome，并通过X射线衍射对其进行了表征，zeta电位和扫描电镜(SEM)。通过SEM和zetasizer测量的脂质体颗粒的尺寸。
■制备的囊泡的表面电荷约为-37mV。针对MRSA分离株的生物膜评估了姜酮和含姜酮的噪声体的作用。此外，使用RealTimePCR评估制备的脂质体对MRSA生物膜基因表达的抗生物膜活性。我们的结果表明，含有姜酮的脂质体的直径为196.1nm，ζ电位为-37.3mV。在1000μg/ml的浓度下，姜酮去除1天和3天龄的MRSA生物膜，而姜酮形成的Niosome在250μg/ml的浓度下去除1、3和5天龄的生物膜，250μg/ml,和500μg/ml。
■结果表明，与游离姜酮相比，含有姜酮的niosome更快地消除了MRSA及其生物膜，这表明姜酮包裹的niosome可以被认为是一种有前途的治疗MRSA及其生物膜的方法。
■研究了从糖尿病溃疡患者中培养的62株MRSA分离物。合成了Niosome，并通过X射线衍射对其进行了表征，zeta电位和扫描电镜(SEM)。通过SEM和zetasizer测量的脂质体颗粒的尺寸。
■制备的囊泡的表面电荷约为-37mV。针对MRSA分离株的生物膜评估了姜酮和含姜酮的噪声体的作用。此外，使用RealTimePCR评估制备的脂质体对MRSA生物膜基因表达的抗生物膜活性。我们的结果表明，含有姜酮的脂质体的直径为196.1nm，ζ电位为-37.3mV。在1000μg/ml的浓度下，姜酮去除1天和3天龄的MRSA生物膜，而姜酮形成的Niosome在250μg/ml的浓度下去除1、3和5天龄的生物膜，250μg/ml,和500μg/ml。
■结果表明，与游离姜酮相比，含有姜酮的niosome更快地消除了MRSA及其生物膜，这表明姜酮包裹的niosome可以被认为是一种有前途的治疗MRSA及其生物膜的方法。
