PDF(Pubmed)
Abstract：
Rapeseed (Brassica napus L.) is an important oilseed crop widely cultivated worldwide, and drought is the main environmental factor limiting its yield enhancement and the expansion of planted areas. SIMILAR TO RCD ONE (SRO) is a plant-specific small gene family that plays a crucial role in plant growth, development, and responses to abiotic stresses such as drought. However, the functional role of SROs in rapeseed remains poorly understood. In this study, 19 BnaSROs were identified from the rapeseed genome, with 9, 10, 10, 18, and 20 members identified from the genomes of Brassica rapa, Brassica nigra, Brassica oleracea, Brassica juncea, and Brassica carinata, respectively. We then analyzed their sequence characteristics, phylogenetic relationships, gene structures, and conserved domains, and explored the collinearity relationships of the SRO members in Brassica napus and Brassica juncea. Next, we focused on the analysis of tissue expression and stress-responsive expression patterns of rapeseed SRO members and examined their expression profiles under ABA, MeJA and water-deficit drought treatments using qPCR. Transcriptome data analysis and qPCR detection indicated that BnaSROs exhibit multiple stress-responsive expression patterns. BnaSRO1 and BnaSRO11, which are likely to function through interactions with NAC transcription factors, were screened as major drought-regulated members. Our results provide a solid foundation for functional analysis of the role of the SRO gene family in abiotic stress responses, especially drought stress responses, in rapeseed.
摘要：
油菜(BrassicanapusL.)是世界范围内广泛种植的重要油料作物,干旱是限制其增产和扩大种植面积的主要环境因子。类似于RCDONE（SRO）是一个植物特异性小基因家族，在植物生长中起着至关重要的作用，发展,以及对干旱等非生物胁迫的反应。然而,SRO在油菜中的功能作用仍然知之甚少。在这项研究中,从油菜基因组中鉴定出19个BnaSRO，从芸苔属基因组中鉴定出9、10、10、18和20个成员，黑油菜,甘蓝,芥菜型油菜，还有CarinataBrassica,分别。然后我们分析了它们的序列特征，系统发育关系，基因结构，和保守的域，并探讨了甘蓝型油菜和芥菜中SRO成员的共线性关系。接下来,我们重点分析了油菜SRO成员的组织表达和应激反应表达模式，并检查了它们在ABA下的表达谱，MeJA和使用qPCR的缺水干旱处理。转录组数据分析和qPCR检测表明BnaSRO表现出多种应激反应表达模式。BnaSRO1和BnaSRO11可能通过与NAC转录因子相互作用而发挥作用，被筛选为主要的干旱调控成员。我们的结果为SRO基因家族在非生物胁迫反应中的作用的功能分析提供了坚实的基础。特别是干旱胁迫响应，油菜籽。
