PDF(Pubmed)
Abstract：
BACKGROUND: Lymphatic metastasis is the major challenge in the treatment of penile cancer. The prognosis of individuals with lymphatic metastasis is extremely poor. Therefore, early identification of disease progression and lymphatic metastasis is an urgent task for researchers in penile cancer worldwide.
METHODS: In this study, using single-cell RNA sequencing, an immune landscape was established for the cancer ecosystem based on 46,861 cells from six patients with penile cancer (four with lymphatic metastasis [stage IV] and two without lymphatic metastasis [stage I]). Using bulk RNA sequencing, the discrepancy between the cancers and their respective metastatic lymph nodes was depicted based on seven patients with penile cancer.
RESULTS: The interaction between epithelial cells, fibroblasts, and endothelial cells, and the functional cooperation among invasion, epithelial-mesenchymal transition, and angiogenesis were found to be important landscapes in the penile cancer ecosystem, playing important roles in progression of cancer and lymph node metastasis.
CONCLUSIONS: This study is the first to investigate the altered tumor microenvironment heterogeneity of penile cancer as it evolves from non-lymphatic to lymphatic metastasis and provides insights into the mechanisms underlying malignant progression, the premetastatic niche, and lymphatic metastasis in penile cancer.
METHODS: In this study, using single-cell RNA sequencing, an immune landscape was established for the cancer ecosystem based on 46,861 cells from six patients with penile cancer (four with lymphatic metastasis [stage IV] and two without lymphatic metastasis [stage I]). Using bulk RNA sequencing, the discrepancy between the cancers and their respective metastatic lymph nodes was depicted based on seven patients with penile cancer.
RESULTS: The interaction between epithelial cells, fibroblasts, and endothelial cells, and the functional cooperation among invasion, epithelial-mesenchymal transition, and angiogenesis were found to be important landscapes in the penile cancer ecosystem, playing important roles in progression of cancer and lymph node metastasis.
CONCLUSIONS: This study is the first to investigate the altered tumor microenvironment heterogeneity of penile cancer as it evolves from non-lymphatic to lymphatic metastasis and provides insights into the mechanisms underlying malignant progression, the premetastatic niche, and lymphatic metastasis in penile cancer.
摘要：
背景：淋巴转移是治疗阴茎癌的主要挑战。具有淋巴转移的个体的预后极差。因此,早期识别疾病进展和淋巴转移是全世界阴茎癌研究人员的一项紧迫任务.
方法：在本研究中，使用单细胞RNA测序，基于来自6例阴茎癌患者的46,861个细胞(4例有淋巴转移[IV期],2例无淋巴转移[I期]),为癌症生态系统建立了免疫景观.使用批量RNA测序，根据7例阴茎癌患者描述了癌症与其各自转移淋巴结之间的差异。
结果：上皮细胞之间的相互作用，成纤维细胞,和内皮细胞，以及入侵之间的功能性合作，上皮-间质转化，发现血管生成是阴茎癌生态系统中的重要景观，在癌症进展和淋巴结转移中起重要作用。
结论：这项研究首次调查阴茎癌从非淋巴转移到淋巴转移时肿瘤微环境异质性的改变，并提供了对恶性进展机制的见解。转移前的生态位，阴茎癌的淋巴转移。
方法：在本研究中，使用单细胞RNA测序，基于来自6例阴茎癌患者的46,861个细胞(4例有淋巴转移[IV期],2例无淋巴转移[I期]),为癌症生态系统建立了免疫景观.使用批量RNA测序，根据7例阴茎癌患者描述了癌症与其各自转移淋巴结之间的差异。
结果：上皮细胞之间的相互作用，成纤维细胞,和内皮细胞，以及入侵之间的功能性合作，上皮-间质转化，发现血管生成是阴茎癌生态系统中的重要景观，在癌症进展和淋巴结转移中起重要作用。
结论：这项研究首次调查阴茎癌从非淋巴转移到淋巴转移时肿瘤微环境异质性的改变，并提供了对恶性进展机制的见解。转移前的生态位，阴茎癌的淋巴转移。
