Abstract：
Bio-based production of silver nanoparticles represents a sustainable alternative to commercially applied physicochemical manufacturing approaches and provides qualitatively highly valuable nanomaterials due to their narrow size dispersity, high stability and biocompatibility with broad application potentials. The intrinsic features of nanoparticles depend on size and shape, whereby the controlled synthesis is a challenging necessity. In the present study, the biosynthesis of size-tuned silver nanoparticles based on cell-free extracts of Saccharomyces cerevisiae DSM 1333 was investigated. Single parameter optimization strategies in phases of cultivation, extraction, and synthesis were performed to modify the nanoparticle scale and yield. Visible light was exploited as a tool in nanoparticle production. The influence of white light on the biosynthesis of silver nanoparticles was determined by using novel LED systems with the exposition of varying irradiation intensities and simultaneous performance of control experiments in the dark. Characterization of the resulting nanomaterials by spectrophotometric analysis, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray spectroscopy, revealed spherical silver nanoparticles with controlled, light-mediated size shifts in markedly increased quantities. Matching of irradiated and non-irradiated reaction mixtures mirrored the enormous functionality of photon input and the high sensitivity of the biosynthesis process. The silver nanoparticle yields increased by more than 90% with irradiation at 1.0 ± 0.2 mW cm - 2 and the reduction of particle dimensions was achieved with significant shifts of size-specific absorption maxima from 440 to 410 nm, corresponding to particle sizes of 130 nm and 100 nm, respectively. White light emerged as an excellent tool for nano-manufacturing with advantageous effects for modulating unique particle properties.
摘要：
银纳米颗粒的生物基生产代表了商业应用的物理化学制造方法的可持续替代方案，并且由于其窄尺寸分散性而提供了质量上非常有价值的纳米材料。高稳定性和生物相容性，具有广阔的应用潜力。纳米粒子的内在特征取决于大小和形状，由此,控制合成是具有挑战性的必要性。在本研究中,研究了基于酿酒酵母DSM1333的无细胞提取物的大小调节的银纳米颗粒的生物合成。栽培阶段的单参数优化策略，提取,和合成进行改性纳米粒子的规模和产量。可见光被用作纳米粒子生产的工具。白光对银纳米颗粒生物合成的影响是通过使用新型LED系统确定的，该系统具有不同的辐照强度和在黑暗中同时进行的对照实验。通过分光光度分析表征所得纳米材料，动态光散射,扫描电子显微镜，和能量色散X射线光谱，揭示了球形银纳米粒子与受控，光介导的大小变化显著增加的数量。辐照和未辐照反应混合物的匹配反映了光子输入的巨大功能和生物合成过程的高灵敏度。在1.0±0.2mWcm-2的照射下，银纳米颗粒的产量增加了90％以上，并且随着尺寸比吸收最大值从440到410nm的显着变化，实现了颗粒尺寸的减小。对应于130nm和100nm的粒径，分别。白光作为用于纳米制造的优异工具出现,具有用于调节独特颗粒性质的有利效果。
