Abstract：
Continuous recombination and variation during replication could lead to rapid evolution and genetic diversity of HIV-1. Some studies had identified that it was easy to develop new recombinant strains of HIV-1 among the populations of men who have sex with men (MSM). Surveillance of genetic variants of HIV-1 in key populations was crucial for comprehending the development of regional HIV-1 epidemics. The finding was reported the identification of two new unique recombinant forms (URF 20110561 and 21110743) from individuals infected with HIV-1 in Tongzhou, Beijing in 2020-2022. Sequences of near full-length genome (NFLG) were amplified, then identification of amplification products used phylogenetic analyses. The result showed that CRF01_AE was the main backbone of 20110561 and 21110743. In the gag region of the virus, 20110561 was inserted two fragments from CRF07_BC, while in the pol and tat regions of the virus, 21110743 was inserted four fragments from CRF07_BC. The CRF01_AE parental origin in the genomes of the two URFs was derived from the CRF01_AE Cluster 4. In the phylogenetic tree, the CRF07_BC parental origin of 20110561 clustered with 07BC_N and the CRF07_BC parental origin of 21110743 clustered with 07BC_O. In summary, the prevalence of novel second-generation URFs of HIV-1 was monitored in Tongzhou, Beijing. The emergence of the novel CRF01_AE/CRF07_BC recombination demonstrated that there was a great significance of continuous monitoring of new URFs in MSM populations to prevent and control the spreading of new HIV-1 URFs.
摘要：
复制过程中的连续重组和变异可能导致HIV-1的快速进化和遗传多样性。一些研究表明，在男男性行为者（MSM）人群中很容易开发新的HIV-1重组株。在关键人群中监测HIV-1的遗传变异对于理解区域HIV-1流行病的发展至关重要。据报道，从通州感染HIV-1的个体中鉴定出两种新的独特重组形式（URF20110561和21110743）。2020-2022年北京。扩增了近全长基因组（NFLG）的序列，然后使用系统发育分析鉴定扩增产物。结果表明，CRF01_AE是20110561和21110743的主要骨架。在病毒的堵嘴区域，20110561插入了来自CRF07_BC的两个片段，而在病毒的pol和tat地区，21110743插入来自CRF07_BC的四个片段。两个URF的基因组中的CRF01_AE亲本起源来源于CRF01_AE簇4。在系统发育树中，20110561的CRF07_BC亲本起源与07BC_N聚集在一起，21110743的CRF07_BC亲本起源与07BC_O聚集在一起。总之,通州对HIV-1新型第二代URFs的流行情况进行了监测，北京。新型CRF01_AE/CRF07_BC重组的出现表明，在MSM人群中持续监测新的URF对于预防和控制新的HIV-1URF的传播具有重要意义。
