关键词: Bloom control Cyanobacteria Potassium Spent nuclear fuel ponds

Mesh : Cyanobacteria / growth & development metabolism physiology Ponds / microbiology Potassium Compounds / pharmacology Hydroxides / pharmacology Potassium / metabolism analysis Biomass

来  源:   DOI:10.1016/j.hal.2024.102654

Abstract:
Microbial blooms have been reported in the First Generation Magnox Storage Pond at the Sellafield Nuclear Facility. The pond is kept alkaline with NaOH to minimise fuel rod corrosion, however alkali-tolerant microbial blooms dominated by the cyanobacterium Pseudanabaena catenata are able to thrive in this hostile environment. This study assessed the impact of alternative alkali-dosing regimens (KOH versus NaOH treatment) on biomass accumulation, using a P. catenata dominated mixed culture, which is representative of the pond environment. Optical density was reduced by 40-67 % with KOH treatment over the 3-month chemostat experiment. Microbial community analysis and proteomics demonstrated that the KOH-dependent inhibition of cell growth was mostly specific to P. catenata. The addition of KOH to nuclear storage ponds may therefore help control growth of this pioneer photosynthetic organism due to its sensitivity to potassium, while maintaining the high pH needed to inhibit the corrosion of stored nuclear fuel.
摘要:
据报道,塞拉菲尔德核设施的第一代Magnox蓄水池中有微生物繁殖。池塘用NaOH保持碱性,以最大程度地减少燃料棒的腐蚀,然而,以蓝细菌假单胞菌为主导的耐碱微生物水华能够在这种恶劣的环境中茁壮成长。本研究评估了替代碱给药方案(KOH与NaOH处理)对生物量积累的影响,使用以P.catenata为主的混合文化,这是池塘环境的代表。在3个月的恒化器实验中,用KOH处理的光密度降低了40-67%。微生物群落分析和蛋白质组学表明,依赖KOH的细胞生长抑制主要是针对P.catenata的。因此,由于对钾的敏感性,向核储存池中添加KOH可能有助于控制这种先驱光合生物的生长,同时保持所需的高pH值,以抑制储存的核燃料的腐蚀。
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