Abstract：
Ovarian cancer, the deadliest gynecological malignancy, primarily treated with chemotherapy. However, systemic chemotherapy often leads to severe toxic side effects and chemoresistance. Drug-loaded aerogels have emerged as a promising method for drug delivery, as they can improve drug solubility and bioavailability, control drug release, and reduce drug distribution in non-targeted tissues, thereby minimizing side effects. In this research, chitosan oligosaccharide (COS)-loaded nanofibers composite chitosan (CS) aerogels (COS-NFs/CS) with a porous network structure were created using nanofiber recombination and freeze-drying techniques. The core layer of the aerogel has a COS loading rate of 60 %, enabling the COS-NFs/CS aerogel to significantly inhibit the migration and proliferation of ovarian cancer cells (resulting in a decrease in the survival rate of ovarian cancer cells to 33.70 % after 48 h). The coaxial fiber\'s unique shell-core structure and the aerogel\'s porous network structure enable the COS-NFs/CS aerogels to release COS steadily and slowly over 30 days, effectively reducing the initial burst release of COS. Additionally, the COS-NFs/CS aerogels exhibit good biocompatibility, degradability (only retaining 18.52 % of their weight after 6 weeks of implantation), and promote angiogenesis, thus promoting wound healing post-oophorectomy. In conclusion, COS-NFs/CS aerogels show great potential for application in the treatment of ovarian cancer.
摘要：
卵巢癌,最致命的妇科恶性肿瘤,主要用化疗治疗。然而,全身化疗常导致严重的毒副作用和化疗耐药。载药气凝胶已成为一种有前途的药物递送方法，因为它们可以提高药物的溶解度和生物利用度，控制药物释放，减少药物在非靶向组织中的分布，从而最大限度地减少副作用。在这项研究中,使用纳米纤维重组和冷冻干燥技术创建了具有多孔网络结构的壳聚糖寡糖（COS）负载的纳米纤维复合壳聚糖（CS）气凝胶（COS-NFs/CS）。气凝胶的芯层具有60%的COS负载率，使COS-NFs/CS气凝胶能够显着抑制卵巢癌细胞的迁移和增殖（导致48小时后卵巢癌细胞的存活率降低至33.70％）。同轴纤维独特的壳-芯结构和气凝胶的多孔网络结构使COS-NFs/CS气凝胶在30天内稳定缓慢地释放COS,有效减少COS的初始爆发释放。此外,COS-NFs/CS气凝胶表现出良好的生物相容性，降解性（植入6周后仅保留其体重的18.52％），促进血管生成，从而促进卵巢切除术后伤口愈合。总之,COS-NFs/CS气凝胶在卵巢癌治疗中显示出巨大的应用潜力。
