Abstract：
With the rise in extreme weather due to global warming, coupled with globalization facilitating the spread of infectious diseases, there\'s a pressing need for portable testing platforms offering simplicity, low cost, and remote transmission, particularly beneficial in resource-limited and non-urban areas. We have developed a portable device using loop-mediated isothermal amplification (LAMP) with spectrometric detection to identify Salmonella Typhimurium DNA. The device utilizes the LinkIt 7697 microcontroller and a microspectrometer to capture and transmit spectral signals in real-time, allowing for improved monitoring and analysis of the reaction progress. We built a hand-held box containing a microspectrometer, thermoelectric cooler, ultraviolet LED, disposable reaction tube, and homemade thermal module, all powered by rechargeable batteries. Additionally, we conducted thorough experiments to ensure temperature accuracy within 1 °C under thermal control, developed a heating module with a LinkIt 7697 IoT development board to heat the DNA mixture to the reaction temperature within 3 min, and integrated foam insulation and a 3D-printed frame to enhance the device\'s thermal stability. We successfully demonstrated the amplification of Salmonella Typhimurium DNA with an impressive sensitivity of 2.83 × 10-4 ng/μL. A remote webpage interface allows for monitoring the temperature and fluorescence during the LAMP process, improving usability. This portable LAMP device with real-time detection offers a cost-effective solution for detecting Salmonella Typhimurium in food products. Its unique design and capabilities make it a promising tool for ensuring food safety.
摘要：
随着全球变暖导致的极端天气的增加，加上全球化促进了传染病的传播，迫切需要提供简单性的便携式测试平台，低成本,和远程传输，特别有利于资源有限和非城市地区。我们已经开发了一种便携式设备，该设备使用环介导等温扩增（LAMP）和光谱检测来鉴定鼠伤寒沙门氏菌DNA。该设备利用LinkIt7697微控制器和微型光谱仪实时捕获和传输光谱信号，允许改进对反应进程的监测和分析。我们建造了一个装有微型光谱仪的手提箱，热电冷却器，紫外线LED,一次性反应管,和自制的热模块，全部由可充电电池供电。此外,我们进行了彻底的实验，以确保在热控制下的温度精度在1°C以内，开发了带有LinkIt7697IoT开发板的加热模块，可在3分钟内将DNA混合物加热至反应温度，集成泡沫绝缘和3D打印框架，以增强设备的热稳定性。我们成功地证明了鼠伤寒沙门氏菌DNA的扩增，其灵敏度为2.83×10-4ng/μL。远程网页界面允许在LAMP过程中监控温度和荧光，提高可用性。这种具有实时检测功能的便携式LAMP设备为检测食品中的鼠伤寒沙门氏菌提供了具有成本效益的解决方案。其独特的设计和功能使其成为确保食品安全的有前途的工具。
