Abstract：
The VITEK®2 AES β-lactam phenotypes of 488 Enterobacterales from North and Latin America generated by the VITEK®2 were compared to the resistance genotypes provided by whole genome sequencing (WGS). The AES provided phenotypic reports for 447 (91.6 %) isolates, including isolates harbouring carbapenemases (195; 43.6 %), ESBLs (103; 23.0 %) and transferable AmpCs (tAmpC; 28; 6.3 %) genes, as well as wildtype isolates (WT; 121; 27.1 %). Overall, the AES report was accurate for 433/447 (96.9 %) isolates. The AES accurately reported carbapenemase, ESBL, and tAmpC phenotypes for 93.7 %, 93.7 %, and 98.4 % of isolates, respectively, and sensitivity/specificity rates were 96.4 %/91.7 %, 98.1 %/92.4 %, 82.1 %/99.5 %, and 100 %/98.8 %. 14 isolates carrying carbapenemase (7 total; 3 KPC, 2 MBL, 2 OXA-48-like), ESBL (2), and tAmpC-encoding genes (5) were not correctly identified by AES. The AES phenotypic report detected resistance mechanisms among Enterobacterales rapidly and could significantly aid future antimicrobial stewardship initiatives and patient care.
摘要：
将由VITEK®2产生的来自北美和拉丁美洲的488例肠杆菌的VITEK®2AESβ-内酰胺表型与通过全基因组测序(WGS)提供的抗性基因型进行比较。AES提供了447个（91.6％）分离株的表型报告，包括含有碳青霉烯酶的分离株(195；43.6%)，ESBLs(103;23.0%)和可转移AmpC(tAmpC;28;6.3%)基因，以及野生型分离株（WT；121；27.1%）。总的来说,AES报告对433/447(96.9%)个分离株准确.AES准确地报告了碳青霉烯酶，ESBL,和tAmpC表型为93.7％，93.7%,和98.4%的分离株，分别,敏感性/特异性分别为96.4%/91.7%,98.1%/92.4%,82.1%/99.5%，和100%/98.8%。14株携带碳青霉烯酶的分离株(共7株；3KPC，2MBL,2OXA-48-like),ESBL(2),和tAmpC编码基因(5)未通过AES正确鉴定。AES表型报告迅速检测到肠杆菌中的耐药机制，可以显着帮助未来的抗菌药物管理计划和患者护理。
