Abstract：
Antibiotic contamination has become an increasingly important environmental problem as a potentially hazardous emergent and recalcitrant pollutant that poses threats to human health. In this study, manganese peroxidase displayed on the outer membrane of Escherichia coli as a whole-cell biocatalyst (E. coli MnP) was expected to degrade antibiotics. The manganese peroxidase activity of the whole-cell biocatalyst was 13.88 ± 0.25 U/L. The typical tetracycline antibiotic chlortetracycline was used to analyze the degradation process. Chlortetracycline at 50 mg/L was effectively transformed via the whole-cell biocatalyst within 18 h. After six repeated batch reactions, the whole-cell biocatalyst retained 87.2 % of the initial activity and retained over 87.46 % of the initial enzyme activity after storage at 25°C for 40 days. Chlortetracycline could be effectively removed from pharmaceutical and livestock wastewater by the whole-cell biocatalyst. Thus, efficient whole-cell biocatalysts are effective alternatives for degrading recalcitrant antibiotics and have potential applications in treating environmental antibiotic contamination.
摘要：
抗生素污染已成为一个日益重要的环境问题，作为一种潜在危险的新兴和顽固的污染物，对人类健康构成威胁。在这项研究中,锰过氧化物酶作为全细胞生物催化剂显示在大肠杆菌的外膜上（E.MncoliP)有望降解抗生素。全细胞生物催化剂的锰过氧化物酶活性为13.88±0.25U/L。采用典型的四环素类抗生素金霉素对其降解过程进行分析。50mg/L的金霉素在18h内通过全细胞生物催化剂有效转化。经过六次重复分批反应，在25°C下储存40天后，全细胞生物催化剂保留了87.2％的初始活性，并保留了超过87.46％的初始酶活性。全细胞生物催化剂可以有效去除制药和畜禽废水中的金霉素。因此，高效的全细胞生物催化剂是降解顽固性抗生素的有效替代品，在治疗环境抗生素污染方面具有潜在的应用价值。
