Abstract：
Disc-like lipid nanoparticles stabilized by saponin biosurfactants display fascinating properties, including their temperature-driven re-organization. β-Aescin, a saponin from seed extract of the horse chestnut tree, shows strong interactions with lipid membranes and has gained interest due to its beneficial therapeutic implications as well as its ability to decompose continuous lipid membranes into size-tuneable discoidal nanoparticles. Here, we characterize lipid nanoparticles formed by aescin and the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine. We present site-resolved insights into central molecular interactions and their modulations by temperature and aescin content. Using the membrane protein bacteriorhodopsin, we additionally demonstrate that, under defined conditions, aescin-lipid discs can accommodate medium-sized transmembrane proteins. Our data reveal the general capability of this fascinating system to generate size-tuneable aescin-lipid-protein particles, opening the road for further applications in biochemical, biophysical and structural studies.
摘要：
由皂苷生物表面活性剂稳定的圆盘状脂质纳米粒显示出令人着迷的特性，包括他们温度驱动的重组。β-七叶皂苷,一种来自栗树种子提取物的皂苷，显示了与脂质膜的强相互作用，并且由于其有益的治疗意义以及将连续脂质膜分解成大小可调的盘状纳米颗粒的能力而引起了人们的兴趣。这里,我们表征了七叶皂苷和磷脂1,2-二肉豆蔻酰-sn-甘油-3-磷酸胆碱形成的脂质纳米颗粒。我们提供了对中心分子相互作用及其受温度和七叶皂苷含量调节的位点解析见解。使用膜蛋白细菌视紫红质，我们还证明，在定义的条件下，七叶皂苷-脂质圆盘可以容纳中等大小的跨膜蛋白。我们的数据揭示了这个迷人的系统产生大小可调的七叶皂苷-脂质-蛋白质颗粒的一般能力，为生化领域的进一步应用开辟了道路，生物物理和结构研究。
