PDF(Pubmed)
Abstract：
During aging and in retinal degenerative diseases, vulnerable retinal pigment epithelial (RPE) cells are subject to mitochondrial dysfunction, creating a need for accessibility to tools which can facilitate assessment of the ocular posterior pole bioenergetics. Here, we present a protocol for quantifying mitochondrial respiration in the posterior eye cup (RPE-choroid-sclera) of young and old mice. We describe steps for eye cup dissection, optimization of tissue size, drug concentrations, and cycle conditions using the XF Cell Mito Stress Test.
摘要：
在衰老和视网膜退行性疾病中，脆弱的视网膜色素上皮（RPE）细胞受到线粒体功能障碍，创造了对工具的可访问性的需求，这些工具可以促进对眼后极生物能量学的评估。这里,我们提出了一种量化年轻和老年小鼠后眼杯中线粒体呼吸（RPE-脉络膜-巩膜）的方案。我们描述了眼杯解剖的步骤，组织大小的优化，药物浓度,和使用XF电池米托应力测试的循环条件。
