Abstract：
Accurately identifying and differentiating the types of injuries in decomposed corpses is a major challenge in forensic identification. Forensic investigations involving decomposed cadavers pose challenges in determining the cause of death. Traditional methods often lack conclusive evidence. However, the implementation of advanced analytical techniques, such as comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GC × GC-TOF/MS), shows promise in overcoming these limitations, but the potential in this area remains limited. Therefore, this study aims to bridge this gap by exploring the potential of GC × GC-TOF/MS in the analysis of volatile organic compounds (VOCs) changes within decaying ante- and post-mortem injuries.The research emphasizes the forensic significance of VOCs changes in decomposed cadavers. We used GC × GC-TOF/MS analysis to identify the specific volatile compounds in putrefied corpse tissue samples from mice. The GC × GC-TOF/MS analysis results showed that under winter conditions, PC1 explained 57.16% of the variance, and PC2 explained 25.23% of the variance; while under summer conditions, PC1 explained 71.89% of the variance, and PC2 explained 24.49% of the variance. This demonstrates the potential of GC × GC-TOF/MS in identifying specific VOCs present in tissue samples that can serve as potential biomarkers for distinguishing between antemortem and postmortem injury. GC × GC-TOF/MS analysis revealed distinct VOC patterns in both conditions. Comprehensive use of GC × GC-TOF/MS analysis enhances accuracy in identifying and characterizing ante- and post-mortem injuries in decomposed cadavers. This study can significantly contribute to the field of forensic medicine and improve the accuracy of forensic investigations.
摘要：
准确识别和区分分解尸体中的伤害类型是法医鉴定的主要挑战。涉及分解尸体的法医调查在确定死亡原因方面提出了挑战。传统方法往往缺乏确凿的证据。然而,实施先进的分析技术，如全面的二维气相色谱-飞行时间质谱（GC×GC-TOF/MS），在克服这些限制方面显示出希望，但这方面的潜力仍然有限。因此,这项研究旨在通过探索GC×GC-TOF/MS在腐烂的死前和死后伤害中挥发性有机化合物（VOCs）变化分析中的潜力来弥合这一差距。该研究强调了分解尸体中VOCs变化的法医学意义。我们使用GC×GC-TOF/MS分析来鉴定小鼠腐烂尸体组织样品中的特定挥发性化合物。GC×GC-TOF/MS分析结果表明,在冬季条件下,PC1解释了57.16%的方差，PC2解释了25.23%的方差；而在夏季条件下，PC1解释了71.89%的方差，PC2解释了24.49%的方差。这证明了GC×GC-TOF/MS在鉴定组织样品中存在的特定VOC方面的潜力，这些VOC可以用作区分死前和死后损伤的潜在生物标志物。GC×GC-TOF/MS分析揭示了两种条件下不同的VOC模式。全面使用GC×GC-TOF/MS分析可提高识别和表征分解尸体的死前和死后伤害的准确性。这项研究可以为法医学领域做出重大贡献，并提高法医调查的准确性。
