Abstract：
BACKGROUND: Oral squamous cell carcinoma (OSCC) is a significant health issue worldwide, and the expression of long non-coding RNAs (lncRNAs) are altered in these malignancies. The present study evaluated the expression level of ATXN1 CDC42EP1 genes and the lncRNAs related to these genes (lnc-ATXN1L, lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1) in paraffin blocks of oral and pharyngeal squamous cell carcinoma (SCC) samples from patients referred to Amir Alam Hospital in Tehran, Iran.
RESULTS: This cross-sectional study was conducted on 76 paraffin blocks of oral and pharyngeal squamous cell carcinoma (SCC) samples from patients referred to Amir Alam Hospital in Tehran. The expression levels of ATXN1, CDC42EP1, lnc-ATXN1L, lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 were measured in all samples using a qPCR Master Mix kit. Real-time PCR was used to perform the reactions, and GAPDH was considered the housekeeping gene. Statistical analyses were conducted utilizing the Statistical Package for the Social Sciences (SPSS) version 22.0. The expression of lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 significantly differed between the two groups. All of them were downregulated (p < 0.05), and no significant difference was observed between the SCC samples and the adjacent tissue in other genes (p > 0.05). The expression of genes was not related to age, sex, size, and tumor location (p > 0.05).
CONCLUSIONS: Dysexpression of lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 can be used for diagnosing OSCC.
RESULTS: This cross-sectional study was conducted on 76 paraffin blocks of oral and pharyngeal squamous cell carcinoma (SCC) samples from patients referred to Amir Alam Hospital in Tehran. The expression levels of ATXN1, CDC42EP1, lnc-ATXN1L, lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 were measured in all samples using a qPCR Master Mix kit. Real-time PCR was used to perform the reactions, and GAPDH was considered the housekeeping gene. Statistical analyses were conducted utilizing the Statistical Package for the Social Sciences (SPSS) version 22.0. The expression of lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 significantly differed between the two groups. All of them were downregulated (p < 0.05), and no significant difference was observed between the SCC samples and the adjacent tissue in other genes (p > 0.05). The expression of genes was not related to age, sex, size, and tumor location (p > 0.05).
CONCLUSIONS: Dysexpression of lnc-ATXN1, lnc-ATXN10, and lnc-CDC42EP1 can be used for diagnosing OSCC.
摘要：
背景：口腔鳞状细胞癌（OSCC）是全球范围内的重要健康问题，长链非编码RNA（lncRNA）的表达在这些恶性肿瘤中发生改变。本研究评估了ATXN1CDC42EP1基因的表达水平和与这些基因相关的lncRNAs(lnc-ATXN1L,lnc-ATXN1，lnc-ATXN10和lnc-CDC42EP1）在转诊至德黑兰阿米尔阿拉姆医院的患者的口腔和咽部鳞状细胞癌（SCC）样品的石蜡块中，伊朗。
结果：这项横断面研究是对来自德黑兰AmirAlam医院的患者的76份口腔和咽部鳞状细胞癌（SCC）石蜡样本进行的。ATXN1、CDC42EP1、lnc-ATXN1L、使用qPCR主混合物试剂盒在所有样品中测量lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1。使用实时PCR进行反应，GAPDH被认为是管家基因。利用社会科学统计软件包(SPSS)22.0版进行统计分析。lnc-ATXN1，lnc-ATXN10和lnc-CDC42EP1的表达在两组之间显着不同。均下调(p<0.05),其他基因在SCC样本和邻近组织之间没有观察到显著差异(p>0.05)。基因的表达与年龄无关，性别,尺寸,和肿瘤位置(p>0.05)。
结论：lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1的表达异常可用于OSCC的诊断。
结果：这项横断面研究是对来自德黑兰AmirAlam医院的患者的76份口腔和咽部鳞状细胞癌（SCC）石蜡样本进行的。ATXN1、CDC42EP1、lnc-ATXN1L、使用qPCR主混合物试剂盒在所有样品中测量lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1。使用实时PCR进行反应，GAPDH被认为是管家基因。利用社会科学统计软件包(SPSS)22.0版进行统计分析。lnc-ATXN1，lnc-ATXN10和lnc-CDC42EP1的表达在两组之间显着不同。均下调(p<0.05),其他基因在SCC样本和邻近组织之间没有观察到显著差异(p>0.05)。基因的表达与年龄无关，性别,尺寸,和肿瘤位置(p>0.05)。
结论：lnc-ATXN1、lnc-ATXN10和lnc-CDC42EP1的表达异常可用于OSCC的诊断。
