METHODS: 120 Fresh tissue and blood samples Forty (40) samples of fresh tissue and blood were collected from patients diagnosed with CRC, twenty (20) samples were collected from a patient diagnosed as healthy. The (qRT-PCR) method is a sensitive technique for the quantifying of steady-state mRNA levels that used to evaluation the expression levels of ID (1-4) gene.
RESULTS: The findings indicate downregulation in ID1 in tissue with a highly significant change between patients and control groups, where upregulation in the ID1 gene is shown in blood samples.ID2 gene also demonstrated high significant change where show upregulation in tissue and downregulation in blood sample. ID3 and ID4 genes show downregulation in tissue and blood samples with a significant change in ID3 blood samples between patient and blood groups.
CONCLUSIONS: Because of the regulation function of the ID family in many processes, the up or down regulation of IDs genes in tumors Proves how important its tumor development, and therefore those proteins can be used as an indicator for CRC.
方法:120个新鲜组织和血液样本从诊断为CRC的患者中收集四十(40)个新鲜组织和血液样本,从诊断为健康的患者中收集了二十(20)个样本。(qRT-PCR)方法是用于定量稳态mRNA水平的灵敏技术,其用于评价ID(1-4)基因的表达水平。
结果:研究结果表明组织中ID1的下调,患者组和对照组之间有非常显著的变化,在血液样本中显示ID1基因的上调。ID2基因还表现出高度显著的变化,其中显示组织中的上调和血液样品中的下调。ID3和ID4基因在组织和血液样品中显示下调,在患者和血型之间ID3血液样品中具有显著变化。
结论:由于ID家族在许多过程中的调节功能,肿瘤中ID基因的上调或下调证明了其在肿瘤发展中的重要性,因此,这些蛋白质可以用作CRC的指标。