Abstract：
BACKGROUND: Recurrent miscarriage (RM) is defined as the occurrence of at least two or three subsequent miscarriages within the 20th -24th weeks of pregnancy. The primary objective of this study was to investigate whether histidine-rich glycoprotein C633T single nucleotide polymorphism (HRG C633T SNP) statistically correlates with the occurrence of RM among Iranian women.
RESULTS: Blood samples from 200 women were taken at the outset of the study. Then, the blood samples of 100 women who had a record of RM (case group) were compared with the other 100 women\'s blood samples who had no record of RM (control group). Following DNA extraction, the polymorphism of histidine-rich glycoprotein C633T (HRG C633T) for every case was specified and all women were genotyped by the amplification-refractory mutation system (ARMS) method. The results of the study revealed that there was a statistically significant difference between T/T genotype (OR = 3.5, CI (1.39-8.77), p = 0.007), and C/T genotype (OR = 1.83, CI (0.99-3.37), p = 0.05) in the case and control groups. Also, a statistically significant association was observed in T allelic frequency in the RM participants compared to the control group (OR = 2.01, CI (1.31-3.09), p = 0.01).
CONCLUSIONS: The present study determined that there was a statistically significant relationship between HRG C633T SNP and increased RM regarding allelic and genotypical aspects. Moreover, it became apparent that women with homozygous T/T genotype were more susceptible to the risk of RM.
RESULTS: Blood samples from 200 women were taken at the outset of the study. Then, the blood samples of 100 women who had a record of RM (case group) were compared with the other 100 women\'s blood samples who had no record of RM (control group). Following DNA extraction, the polymorphism of histidine-rich glycoprotein C633T (HRG C633T) for every case was specified and all women were genotyped by the amplification-refractory mutation system (ARMS) method. The results of the study revealed that there was a statistically significant difference between T/T genotype (OR = 3.5, CI (1.39-8.77), p = 0.007), and C/T genotype (OR = 1.83, CI (0.99-3.37), p = 0.05) in the case and control groups. Also, a statistically significant association was observed in T allelic frequency in the RM participants compared to the control group (OR = 2.01, CI (1.31-3.09), p = 0.01).
CONCLUSIONS: The present study determined that there was a statistically significant relationship between HRG C633T SNP and increased RM regarding allelic and genotypical aspects. Moreover, it became apparent that women with homozygous T/T genotype were more susceptible to the risk of RM.
摘要：
背景：复发性流产（RM）定义为在妊娠20-24周内发生至少2或3次随后的流产。这项研究的主要目的是调查富含组氨酸的糖蛋白C633T单核苷酸多态性（HRGC633TSNP）是否与伊朗妇女RM的发生具有统计学相关性。
结果：在研究开始时采集了200名女性的血液样本。然后，将100例有RM记录的女性(病例组)的血液样本与其他100例无RM记录的女性(对照组)的血液样本进行比较.DNA提取后,确定了每个病例的富含组氨酸糖蛋白C633T(HRGC633T)的多态性,并通过扩增-难治性突变系统(ARMS)方法对所有女性进行基因分型.研究结果表明，T/T基因型之间存在统计学上的显着差异（OR=3.5，CI（1.39-8.77），p=0.007),和C/T基因型（OR=1.83，CI（0.99-3.37），在病例组和对照组中p=0.05）。此外，与对照组相比，RM参与者的T等位基因频率具有统计学意义（OR=2.01，CI（1.31-3.09），p=0.01)。
结论：本研究确定，在等位基因和基因型方面，HRGC633TSNP和RM增加之间存在统计学上显著的关系。此外，很明显,具有纯合T/T基因型的女性更容易患RM.
结果：在研究开始时采集了200名女性的血液样本。然后，将100例有RM记录的女性(病例组)的血液样本与其他100例无RM记录的女性(对照组)的血液样本进行比较.DNA提取后,确定了每个病例的富含组氨酸糖蛋白C633T(HRGC633T)的多态性,并通过扩增-难治性突变系统(ARMS)方法对所有女性进行基因分型.研究结果表明，T/T基因型之间存在统计学上的显着差异（OR=3.5，CI（1.39-8.77），p=0.007),和C/T基因型（OR=1.83，CI（0.99-3.37），在病例组和对照组中p=0.05）。此外，与对照组相比，RM参与者的T等位基因频率具有统计学意义（OR=2.01，CI（1.31-3.09），p=0.01)。
结论：本研究确定，在等位基因和基因型方面，HRGC633TSNP和RM增加之间存在统计学上显著的关系。此外，很明显,具有纯合T/T基因型的女性更容易患RM.
