Abstract：
Gravid culicine mosquitoes rely on olfactory cues for selecting breeding sites containing organic detritus. While this capacity of the mosquitoes is used for surveillance and control, the current methodology is unwieldy, unreliable and expensive in time and labour. This study evaluated the dose-dependent attraction and oviposition response of gravid Culex quinquefasciatus to alfalfa infusions. Through combined chemical and electrophysiological analyses, bioactive volatile organic compounds (VOCs) in the headspace of alfalfa infusions, eliciting attraction, were identified. While phenolic and indolic compounds were the most abundant bioactive VOCs, additional VOCs, including a monoterpene, were required to elicit a significant behavioural response to the synthetic odour blend of alfalfa infusions. Comparative analysis with the commercially available mosquito oviposition pheromone (MOP) was also conducted demonstrating that this standardised synthetic alfalfa infusion odour blend offers a promising lure for targeted surveillance and control of Culex mosquitoes, which may contribute to disease prevention and public health protection.
摘要：
Gravidculicine蚊子依靠嗅觉线索来选择含有有机碎屑的繁殖地点。虽然蚊子的这种能力用于监视和控制，目前的方法是笨拙的，不可靠和昂贵的时间和劳动力。这项研究评估了妊娠库蚊对苜蓿输注的剂量依赖性吸引力和产卵反应。通过结合化学和电生理分析，苜蓿输液顶部空间中的生物活性挥发性有机化合物（VOCs），引发吸引力，已确定。虽然酚类化合物和吲哚类化合物是最丰富的生物活性VOCs，额外的VOCs,包括单萜，需要引起对苜蓿输液的合成气味混合物的显着行为反应。还进行了与市售蚊子产卵信息素（MOP）的比较分析，证明了这种标准化的合成苜蓿输液气味混合物为有针对性地监视和控制库蚊提供了有希望的诱饵。这可能有助于疾病预防和公共卫生保护。
