PDF(Pubmed)
Abstract：
\"Background/Aim\": the current inability to diagnose Pancreatic Cancer Adenocarcinoma (PDAC) at an early stage strongly influences therapeutic strategies. Protein Induced by Vitamin K Absence (PIVKA II) showed an accurate diagnostic performance for PDAC. Since circulating PIVKA II has been recently associated with pancreatic origin cells with Vimentin, an epithelial-to-mesenchymal transition (EMT) early activation marker, the aim of this study was to investigate in vivo the combination between the two proteins. \"Materials and Methods\": we assayed the presence of PIVKA II and Vimentin proteins by using different diagnostic methods. A total of 20 PDAC patients and 10 healthy donors were tested by Western Blot analysis; 74 PDAC patient and 46 healthy donors were assayed by ECLIA and Elisa. \"Results\": Western Blot analysis showed the concomitant expression of PIVKA II and Vimentin in PDAC patient sera. Immunometric assay performed on a larger cohort of patients demonstrated that 72% of PIVKA II-positive PDAC patients were Vimentin-positive. Additionally, in a group of PDAC patients with PIVKA II levels ≥2070 ng/mL, the percentage of Vimentin-positive subjects reached 84%. \"Conclusion\": the association between PIVKA II protein and the EMT suggests that this molecule could be considered a marker of the acquisition of an aggressive phenotype.
摘要：
“背景/目的”：目前无法在早期诊断胰腺癌腺癌（PDAC）强烈影响治疗策略。维生素K缺乏诱导的蛋白质（PIVKAII）对PDAC具有准确的诊断性能。由于循环PIVKAII最近与具有波形蛋白的胰腺起源细胞相关，上皮-间质转化（EMT）早期激活标记，这项研究的目的是在体内研究两种蛋白质之间的组合。“材料和方法”：我们使用不同的诊断方法测定了PIVKAII和波形蛋白的存在。通过Western印迹分析测试了总共20名PDAC患者和10名健康供体;通过ECLIA和Elisa分析了74名PDAC患者和46名健康供体。“结果”：蛋白质印迹分析显示PDAC患者血清中PIVKAII和波形蛋白的同时表达。对更大的患者队列进行的免疫分析显示，72％的PIVKAII阳性PDAC患者为波形蛋白阳性。此外,在一组PIVKAII水平≥2070ng/mL的PDAC患者中，波形蛋白阳性受试者的百分比达到84%。“结论”：PIVKAII蛋白与EMT之间的关联表明，该分子可被认为是获得侵袭性表型的标志。
