PDF(Pubmed)
Abstract：
The present study aimed to extract nanocellulose (NC) from sugarcane bagasse agricultural waste through a chemical method (sulfuric acid hydrolysis and ultrasonication). Subsequently, the nanocellulose product was conjugated with polylysine (NC-PL) and assessed for its efficacy in reducing the toxicity of Fumonisin B1 (FB1), a mycotoxin produced by fungi commonly found in corn, wheat, and other grains. Experimental results confirmed the successful conjugation of NC and PL, as evidenced by FTIR peaks at 1635 and 1625 cm-1 indicating amide I and amide II vibrations in polylysine (PL). SEM analysis revealed a larger size due to PL coating, consistent with DLS results showing the increased size and positive charge (38.0 mV) on the NC-PL surface. Moreover, the effect of FB1 adsorption by NC and NC-PL was evaluated at various concentrations (0-200,000 μg/mL). NC-PL demonstrated the ability to adsorb FB1 at concentrations of 2000, 20,000, and 200,000 μg/mL, with adsorption efficiencies of 94.4-100%. Human hepatocellular carcinoma (HepG2) cells were utilized to assess NC and NC-PL cytotoxic effects. This result is a preliminary step towards standardizing results for future studies on their application as novel FB1 binders in food, food packaging, and functional feeds.
摘要：
本研究旨在通过化学方法（硫酸水解和超声处理）从甘蔗渣农业废物中提取纳米纤维素（NC）。随后，将纳米纤维素产品与聚赖氨酸（NC-PL）结合，并评估其降低FumononinB1（FB1）毒性的功效，一种真菌毒素，由玉米中常见的真菌产生，小麦,和其他谷物。实验结果证实了NC和PL的成功共轭，如1635和1625cm-1处的FTIR峰所证明的,表明聚赖氨酸(PL)中的酰胺I和酰胺II振动。SEM分析显示，由于PL涂层，尺寸较大，与DLS结果一致，显示NC-PL表面上的尺寸和正电荷（38.0mV）增加。此外，在各种浓度（0-200,000μg/mL）下评估了NC和NC-PL对FB1吸附的影响。NC-PL证明了在2000、20,000和200,000μg/mL浓度下吸附FB1的能力，吸附效率为94.4-100%。人肝细胞癌(HepG2)细胞用于评估NC和NC-PL细胞毒性作用。该结果是标准化结果的初步步骤，以便将来研究其作为食品中新型FB1粘合剂的应用。食品包装,和功能性饲料。
