PDF(Pubmed)
Abstract：
Cerium oxide nanoparticles (CeONPs), as part of tissue regeneration matrices, can protect cells from reactive oxygen species and oxidative stress. In addition, they can influence the properties of the scaffold, including its electrospinnability and mechanical strength. In this work, we prepared electrospun fiber mats from a chitosan and polyethylene oxide blend (CS-PEO) with the addition of ceria nanoparticles (CS-PEO-CeONP). The addition of CeONPs resulted in a smaller fiber diameter and higher swelling compared to CS-PEO fiber mats. CeONP-modified fiber mats also had a higher Young\'s modulus due to the reinforcing effect of the nanoparticles. Both mats had comparable adhesion and cytocompatibility to mesenchymal stem cells, which had a more rounded morphology on CS-PEO-CeONP compared to elongated cells on the CS-PEO mats. Biocompatibility in an in vivo rat model showed no acute toxicity, no septic or allergic inflammation, and no rough scar tissue formation. The degradation of both mats passed the stage of matrix swelling. CS-PEO-CeONP showed significantly slower biodegradation, with most of the matrix remaining in the tissue after 90 days. The reactive inflammation was aseptic in nature with the involvement of multinucleated foreign-body type giant cells and was significantly reduced by day 90. CeONPs induced the formation of the implant\'s connective tissue capsule. Thus, the introduction of CeONPs influenced the physicochemical properties and biological activity of CS-PEO nanofiber mats.
摘要：
氧化铈纳米粒子(CeONPs),作为组织再生基质的一部分，可以保护细胞免受活性氧和氧化应激。此外,它们可以影响支架的性能，包括其电纺丝性和机械强度。在这项工作中,我们从壳聚糖和聚环氧乙烷共混物（CS-PEO）中加入二氧化铈纳米颗粒（CS-PEO-CeONP）制备了电纺纤维垫。与CS-PEO纤维毡相比,CeONPs的添加导致更小的纤维直径和更高的溶胀。由于纳米颗粒的增强作用,CeONP改性的纤维毡也具有较高的杨氏模量。两种垫子都与间充质干细胞具有可比的粘附性和细胞相容性，与CS-PEO垫上的细长细胞相比,其在CS-PEO-CeONP上具有更圆形的形态。体内大鼠模型的生物相容性无急性毒性,没有败血症或过敏性炎症，没有粗糙的疤痕组织形成.两个垫的降解都经过了基质溶胀的阶段。CS-PEO-CeONP显示出显著较慢的生物降解，90天后大部分基质保留在组织中。反应性炎症本质上是无菌的，涉及多核异物型巨细胞，到第90天显着减少。CeONPs诱导植入物结缔组织包膜的形成。因此，CeONPs的引入影响了CS-PEO纳米纤维垫的理化性质和生物活性。
