PDF(Pubmed)
Abstract：
The dental implant surface plays a crucial role in osseointegration. The topography and physicochemical properties will affect the cellular functions. In this research, four distinct titanium surfaces have been studied: machined acting (MACH), acid etched (AE), grit blasting (GBLAST), and a combination of grit blasting and subsequent acid etching (GBLAST + AE). Human amniotic mesenchymal (hAMSCs) and epithelial stem cells (hAECs) isolated from the amniotic membrane have attractive stem-cell properties. They were cultured on titanium surfaces to analyze their impact on biological behavior. The surface roughness, microhardness, wettability, and surface energy were analyzed using interferometric microscopy, Vickers indentation, and drop-sessile techniques. The GBLAST and GBLAST + AE surfaces showed higher roughness, reduced hydrophilicity, and lower surface energy with significant differences. Increased microhardness values for GBLAST and GBLAST + AE implants were attributed to surface compression. Cell viability was higher for hAMSCs, particularly on GBLAST and GBLAST + AE surfaces. Alkaline phosphatase activity enhanced in hAMSCs cultured on GBLAST and GBLAST + AE surfaces, while hAECs showed no mineralization signals. Osteogenic gene expression was upregulated in hAMSCs on GBLAST surfaces. Moreover, α2 and β1 integrin expression enhanced in hAMSCs, suggesting a surface-integrin interaction. Consequently, hAMSCs would tend toward osteoblastic differentiation on grit-blasted surfaces conducive to osseointegration, a phenomenon not observed in hAECs.
摘要：
牙种植体表面在骨整合中起着至关重要的作用。形貌和物理化学性质将影响细胞功能。在这项研究中,已经研究了四个不同的钛表面：加工作用(MACH)，酸蚀（AE），喷砂(GBLAST),以及喷砂和随后的酸蚀刻(GBLAST+AE)的组合。从羊膜分离的人羊膜间充质干细胞(hAMSCs)和上皮干细胞(hAECs)具有吸引人的干细胞特性。将它们培养在钛表面以分析其对生物学行为的影响。表面粗糙度,显微硬度,润湿性,使用干涉显微镜分析表面能，维克斯压痕，和下降固着技术。GBLAST和GBLAST+AE表面显示出更高的粗糙度，降低亲水性，和较低的表面能具有显著差异。GBLAST和GBLAST+AE植入物的显微硬度值增加归因于表面压缩。hAMSCs的细胞活力更高，特别是在GBLAST和GBLAST+AE表面上。在GBLAST和GBLASTAE表面培养的hAMSCs中碱性磷酸酶活性增强，而hAECs没有显示矿化信号。成骨基因表达在gBLAST表面的hAMSCs中上调。此外，α2和β1整合素在hAMSCs中的表达增强,表明表面整合素相互作用。因此,hAMSCs倾向于在喷砂表面上向成骨细胞分化，有利于骨整合，在HAECs中未观察到的现象。
