PDF(Pubmed)
Abstract：
Gastric cancer is the fifth most common disease in the world and the fourth most common cause of death. It is diagnosed through esophagogastroduodenoscopy with biopsy; however, there are limitations in finding lesions in the early stages. Recently, research has been actively conducted to use liquid biopsy to diagnose various cancers, including gastric cancer. Various substances derived from cancer are reflected in the blood. By analyzing these substances, it was expected that not only the presence or absence of cancer but also the type of cancer can be diagnosed. However, the amount of these substances is extremely small, and even these have various variables depending on the characteristics of the individual or the characteristics of the cancer. To overcome these, we collected methylated DNA fragments using MeDIP and compared them with normal plasma to characterize gastric cancer tissue or patients\' plasma. We attempted to diagnose gastric cancer using the characteristics of cancer reflected in the blood through the cancer tissue and patients\' plasma. As a result, we confirmed that the consistency of common methylated fragments between tissue and plasma was approximately 41.2% and we found the possibility of diagnosing and characterizing cancer using the characteristics of the fragments through SFR and 5\'end-motif analysis.
摘要：
胃癌是世界上第五大常见疾病和第四大最常见的死亡原因。它是通过食管胃十二指肠镜检查和活检诊断的；然而，早期发现病变存在局限性.最近,已经积极进行了使用液体活检来诊断各种癌症的研究，包括胃癌.来自癌症的各种物质反映在血液中。通过分析这些物质，预计不仅可以诊断癌症的存在或不存在，而且可以诊断癌症的类型。然而,这些物质的量非常小，甚至这些变量取决于个体的特征或癌症的特征。为了克服这些，我们使用MeDIP收集甲基化DNA片段,并将其与正常血浆进行比较,以确定胃癌组织或患者血浆的特征.我们试图利用通过癌组织和患者血浆反映在血液中的癌症特征来诊断胃癌。因此,我们证实了组织和血浆之间常见甲基化片段的一致性约为41.2%,我们发现通过SFR和5'端基序分析,使用片段的特征诊断和表征癌症的可能性.
