PDF(Pubmed)
Abstract：
Pollen, in addition to allergens, comprise low molecular weight components (LMC) smaller than 3 kDa. Emerging evidence indicates the relevance of LMC in allergic immune responses. However, the interaction of birch pollen (BP)-derived LMC and epithelial cells has not been extensively studied. We investigated epithelial barrier modifications induced by exposure to BP LMC, using the human bronchial epithelial cell line 16HBE14o-. Epithelial cell monolayers were apically exposed to the major BP allergen Bet v 1, aqueous BP extract or BP-derived LMC. Barrier integrity after the treatments was monitored by measuring transepithelial electrical resistance at regular intervals and by using the xCELLigence Real-Time Cell Analysis system. The polarized release of cytokines 24 h following treatment was measured using a multiplex immunoassay. Epithelial barrier integrity was significantly enhanced upon exposure to BP LMC. Moreover, BP LMC induced the repair of papain-mediated epithelial barrier damage. The apical release of CCL5 and TNF-α was significantly reduced after exposure to BP LMC, while the basolateral release of IL-6 significantly increased. In conclusion, the results of our study demonstrate that BP-derived LMC modify the physical and immunological properties of bronchial epithelial cells and thus regulate airway epithelial barrier responses.
摘要：
花粉,除了过敏原，包含小于3kDa的低分子量组分(LMC)。新出现的证据表明LMC在过敏性免疫应答中的相关性。然而,桦树花粉（BP）来源的LMC与上皮细胞的相互作用尚未得到广泛研究。我们调查了暴露于BPLMC诱导的上皮屏障修饰，使用人支气管上皮细胞系16HBE14-。将上皮细胞单层顶部暴露于主要的BP过敏原Betv1，水性BP提取物或BP衍生的LMC。通过定期测量跨上皮电阻并使用xCELLigence实时细胞分析系统来监测处理后的屏障完整性。使用多重免疫测定法测量治疗后24小时细胞因子的极化释放。暴露于BPLMC后，上皮屏障完整性显着增强。此外，BPLMC诱导木瓜蛋白酶介导的上皮屏障损伤修复。暴露于BPLMC后，CCL5和TNF-α的顶端释放显着降低，而基底外侧释放的IL-6显著增加。总之,我们的研究结果表明，BP衍生的LMC改变了支气管上皮细胞的物理和免疫特性，从而调节了气道上皮屏障反应。
