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Abstract：
Nematodes of the genus Trichinella are important pathogens of humans and animals. This study aimed to enhance the genomic and transcriptomic resources for T. pseudospiralis (non-encapsulated phenotype) and T. spiralis (encapsulated phenotype) and to explore transcriptional profiles. First, we improved the assemblies of the genomes of T. pseudospiralis (code ISS13) and T. spiralis (code ISS534), achieving genome sizes of 56.6 Mb (320 scaffolds, and an N50 of 1.02 Mb) and 63.5 Mb (568 scaffolds, and an N50 value of 0.44 Mb), respectively. Then, for each species, we produced RNA sequence data for three key developmental stages (first-stage muscle larvae [L1s], adults, and newborn larvae [NBLs]; three replicates for each stage), analysed differential transcription between stages, and explored enriched pathways and processes between species. Stage-specific upregulation was linked to cellular processes, metabolism, and host-parasite interactions, and pathway enrichment analysis showed distinctive biological processes and cellular localisations between species. Indeed, the secreted molecules calmodulin, calreticulin, and calsyntenin-with possible roles in modulating host immune responses and facilitating parasite survival-were unique to T. pseudospiralis and not detected in T. spiralis. These insights into the molecular mechanisms of Trichinella-host interactions might offer possible avenues for developing new interventions against trichinellosis.
摘要：
旋毛虫属线虫是人类和动物的重要病原体。本研究旨在增强假螺旋藻（非包封表型）和螺旋藻（包封表型）的基因组和转录组资源，并探索转录谱。首先,我们改进了假螺旋藻（代码ISS13）和螺旋藻（代码ISS534）基因组的组装，实现56.6Mb的基因组大小(320个支架，N50为1.02Mb)和63.5Mb(568个脚手架，N50值为0.44Mb)，分别。然后，对于每个物种，我们产生了三个关键发育阶段的RNA序列数据(第一阶段肌肉幼虫[L1s]，成年人,和新生幼虫[NBLs]；每个阶段重复三次)，分析了阶段之间的差异转录，探索物种之间丰富的路径和过程。阶段特异性上调与细胞过程有关，新陈代谢，和宿主-寄生虫相互作用，和途径富集分析显示了物种之间独特的生物过程和细胞定位。的确,分泌的分子钙调蛋白，钙网蛋白,和calsyntenin-可能在调节宿主免疫反应和促进寄生虫存活中起作用-是假螺旋藻特有的，在螺旋藻中未检测到。这些对旋毛虫与宿主相互作用的分子机制的见解可能为开发针对旋毛虫病的新干预措施提供可能的途径。
