PDF(Pubmed)
Abstract：
This study investigated the impact of adding hydroxyapatite nanoparticles to implant surfaces treated with zirconia blasting and acid etching (ZiHa), focusing on structural changes and bone healing parameters in low-density bone sites. The topographical characterization of titanium discs with a ZiHa surface and a commercially modified zirconia-blasted and acid-etched surface (Zi) was performed using scanning electron microscopy, profilometry, and surface-free energy. For the in vivo assessment, 22 female rats were ovariectomized and kept for 90 days, after which one implant from each group was randomly placed in each tibial metaphysis of the animals. Histological and immunohistochemical analyses were performed at 14 and 28 days postoperatively (decalcified lab processing), reverse torque testing was performed at 28 days, and histometry from calcified lab processing was performed at 60 days The group ZiHa promoted changes in surface morphology, forming evenly distributed pores. For bone healing, ZiHa showed a greater reverse torque, newly formed bone area, and bone/implant contact values compared to group Zi (p < 0.05; t-test). Qualitative histological and immunohistochemical analyses showed higher features of bone maturation for ZiHa on days 14 and 28. This preclinical study demonstrated that adding hydroxyapatite to zirconia-blasted and acid-etched surfaces enhanced peri-implant bone healing in ovariectomized rats. These findings support the potential for improving osseointegration of dental implants, especially in patients with compromised bone metabolism.
摘要：
这项研究调查了添加羟基磷灰石纳米颗粒到经过氧化锆喷砂和酸蚀刻（ZiHa）处理的植入物表面的影响，关注低密度骨部位的结构变化和骨愈合参数。使用扫描电子显微镜对具有ZiHa表面和商业改性的氧化锆喷砂和酸蚀刻表面（Zi）的钛圆盘进行了形貌表征，轮廓术,和表面自由能。对于体内评估，22只雌性大鼠切除卵巢，饲养90天，之后，将每组的一个植入物随机放置在动物的每个胫骨干and端。在术后14天和28天进行组织学和免疫组织化学分析（脱钙实验室处理），反向扭矩测试在28天进行，和组织学从钙化实验室处理在60天进行ZiHa组促进表面形态的变化，形成均匀分布的孔隙。为了骨骼愈合，ZiHa表现出更大的反向扭矩，新形成的骨骼区域，和骨/植入物接触值与Zi组相比(p<0.05;t检验)。定性组织学和免疫组织化学分析显示，第14天和第28天ZiHa的骨成熟特征更高。这项临床前研究表明，在氧化锆喷砂和酸蚀表面添加羟基磷灰石可增强卵巢切除大鼠的种植体周围骨愈合。这些发现支持了改善牙科植入物骨整合的潜力，尤其是骨代谢受损的患者。
