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Abstract：
As a widely distributed plant in Northeast China, Carex meyeriana Kunth (CMK) is generally considered to have antibacterial properties; however, there is a lack of scientific evidence for this. Therefore, we investigated the chemical composition of CMK extract and its effect against C. albicans. A total of 105 compounds were identified in the alcohol extracts of CMK by UPLC-Q-TOF-MS. Most were flavonoids, with Luteolin being the most represented. Among them, 19 compounds are found in the C. albicans lysates. After treatment with CMK ethanol extract, a significant reduction in the number of C. albicans colonies was observed in a vaginal douche solution from day 5 (p < 0.05). Furthermore, the CMK extract can reduce the number of C. albicans spores. The levels of IL-4, IL-6, IL-10, IL-1β, and TNF-α in vaginal tissues all exhibited a significant decrease (p < 0.05) compared to those in the model group as determined by ELISA. The results of HE staining showed that CMK extract can eliminate vaginal mucosa inflammation. CMK adjusts the vaginal mucosa cells by targeting twenty-six different metabolites and five specific metabolic pathways in order to effectively eliminate inflammation. Simultaneously, the CMK regulates twenty-three types of metabolites and six metabolic pathways against C. albicans infection. So, CMK strongly inhibits the growth of C. albicans and significantly reduces vaginal inflammation, making it a promising candidate for treating C. albicans infection.
摘要：
作为东北地区分布广泛的植物,CarexmeyerianaKunth（CMK）通常被认为具有抗菌性能；然而，这方面缺乏科学证据。因此,我们研究了CMK提取物的化学成分及其对白色念珠菌的影响。通过UPLC-Q-TOF-MS在CMK的醇提取物中鉴定出总共105种化合物。大多数是类黄酮，木犀草素是最有代表性的。其中,在白色念珠菌裂解物中发现了19种化合物。用CMK乙醇提取物处理后，从第5天开始,在阴道冲洗液中观察到白色念珠菌菌落数量显著减少(p<0.05)。此外，CMK提取物可以减少白色念珠菌孢子的数量。IL-4、IL-6、IL-10、IL-1β、通过ELISA测定，与模型组相比，阴道组织中的TNF-α和TNF-α均表现出显着降低（p<0.05）。HE染色成果显示CMK提取物能消除阴道粘膜炎症。CMK通过靶向二十六个不同的代谢物和五个特定的代谢途径来调节阴道粘膜细胞,以有效地消除炎症。同时,CMK调节针对白色念珠菌感染的二十三种代谢物和六种代谢途径。所以,CMK强烈抑制白色念珠菌的生长，并显著减少阴道炎症，使其成为治疗白色念珠菌感染的有希望的候选者。
