PDF(Pubmed)
Abstract：
Persimmon (Diospyros kaki Thunb.) fruit size variation is abundant. Studying the size of the persimmon fruit is helpful in improving its economic value. At present, the regulatory mechanism of persimmon fruit size formation is still unclear. In this study, the mechanism of fruit size formation was investigated through morphological, cytological and transcriptomic analyses, as well as exogenous ethrel and aminoethoxyinylglycine (AVG: ethylene inhibitor) experiments using the large fruit and small fruit of \'Yaoxianwuhua\'. The results showed that stages 3-4 (June 11-June 25) are the crucial morphological period for differentiation of large fruit and small fruit in persimmon. At this crucial morphological period, the cell number in large fruit was significantly more than that in small fruit, indicating that the difference in cell number is the main reason for the differentiation of persimmon fruit size. The difference in cell number was caused by cell division. CNR1, ANT, LAC17 and EB1C, associated with cell division, may be involved in regulating persimmon fruit size. Exogenous ethrel resulted in a decrease in fruit weight, and AVG treatment had the opposite effect. In addition, LAC17 and ERF114 were upregulated after ethrel treatment. These results indicated that high ethylene levels can reduce persimmon fruit size, possibly by inhibiting cell division. This study provides valuable information for understanding the regulation mechanism of persimmon fruit size and lays a foundation for subsequent breeding and artificial regulation of fruit size.
摘要：
柿子（DiospyroskakiThunb。)果实大小变化丰富。研究柿子果实的大小有助于提高其经济价值。目前,柿子果实大小形成的调控机制尚不清楚。在这项研究中,通过形态学研究了果实大小形成的机理，细胞学和转录组学分析，以及使用“Yaoxianwuhua”的大果实和小果实进行的外源乙烯和氨基乙氧基甘氨酸（AVG：乙烯抑制剂）实验。结果表明，3-4阶段（6月11日至6月25日）是柿子大果和小果分化的关键形态时期。在这个关键的形态学时期，大果实中的细胞数量明显多于小果实中的细胞数量，说明细胞数量的差异是柿子果实大小分化的主要原因。细胞数量的差异是由细胞分裂引起的。CNR1,ANT,LAC17和EB1C，与细胞分裂有关，可能参与调节柿子果实的大小。外源性乙酮导致水果重量减少，和AVG治疗有相反的效果。此外,LAC17和ERF114在乙素治疗后上调。这些结果表明，高乙烯水平可以降低柿子果实的大小，可能是通过抑制细胞分裂.本研究为理解柿子果实大小的调控机制提供了有价值的信息,为后续育种和人工调控果实大小奠定了基础。
