PDF(Pubmed)
Abstract：
Two cases of complicated pain exist: posterior screw fixation and myofascial pain. Intramuscular pulsed radiofrequency (PRF) may be an alternative treatment for such patients. This is a two-stage animal study. In the first stage, two muscle groups and two nerve groups were subdivided into a high-temperature group with PRF at 58 °C and a regular temperature with PRF at 42 °C in rats. In the second stage, two nerve injury groups were subdivided into nerve injury with PRF 42 °C on the sciatic nerve and muscle. Blood and spinal cord samples were collected. In the first stage, the immunohistochemical analysis showed that PRF upregulated brain-derived neurotrophic factor (BDNF) in the spinal cord in both groups of rats. In the second stage, the immunohistochemical analysis showed significant BDNF and tropomyosin receptor kinase B (TrkB) expression within the spinal cord after PRF in muscles and nerves after nerve injury. The blood biomarkers showed a significant increase in BDNF levels. PRF in the muscle in rats could upregulate BDNF-TrkB in the spinal cord, similar to PRF on the sciatica nerve for pain relief in rats. PRF could be considered clinically for patients with complicated pain and this study also demonstrated the role of BDNF in pain modulation. The optimal temperature for PRF was 42 °C.
摘要：
存在2例复杂疼痛：后路螺钉固定和肌筋膜疼痛。肌内脉冲射频（PRF）可能是此类患者的替代治疗方法。这是一个两阶段的动物研究。在第一阶段,在大鼠中，将两个肌肉组和两个神经组细分为58°C的PRF高温组和42°C的PRF常规温度。在第二阶段,将两个神经损伤组细分为坐骨神经和肌肉PRF42°C的神经损伤。收集血液和脊髓样品。在第一阶段,免疫组织化学分析显示,PRF对两组大鼠脊髓脑源性神经营养因子(BDNF)均有上调作用。在第二阶段,免疫组织化学分析显示，神经损伤后PRF后，肌肉和神经中的BDNF和原肌球蛋白受体激酶B（TrkB）在脊髓内明显表达。血液生物标志物显示BDNF水平显著增加。大鼠肌肉中的PRF可以上调脊髓中的BDNF-TrkB，与大鼠坐骨神经痛缓解疼痛的PRF相似。PRF可以在临床上考虑用于患有复杂疼痛的患者，并且该研究还证明了BDNF在疼痛调节中的作用。PRF的最佳温度为42°C。
