PDF(Pubmed)
Abstract：
Acute lung injury (ALI) is a condition associated with acute respiratory failure, resulting in significant morbidity and mortality. It involves cellular changes such as disruption of the alveolar-capillary membrane, excessive neutrophil migration, and release of inflammatory mediators. Broncho-Vaxom® (BV), a lyophilized product containing cell membrane components derived from eight bacteria commonly found in the respiratory tract, is known for its potential to reduce viral and bacterial lung infections. However, the specific effect of BV on ALI has not been clearly defined. This study explored the preventive effects of BV and its underlying mechanisms in a lipopolysaccharide (LPS)-induced ALI mouse model. Oral BV (1 mg/kg) gavage was administered one hour before the intratracheal injection of LPS to evaluate its preventive effect on the ALI model. The pre-administration of BV significantly mitigates inflammatory parameters, including the production of inflammatory mediators, macrophage infiltration, and NF-κB activation in lung tissue, and the increase in inflammatory cells in bronchoalveolar lavage fluid (BALF). Moreover, BV (3 μg/mL) pretreatment reduced the expression of M1 macrophage markers, interleukins (IL-1β, IL-6), tumor necrosis factor α, and cyclooxygenase-2, which are activated by LPS, in both mouse alveolar macrophage MH-S cells and human macrophage THP-1 cells. These findings showed that BV exhibits anti-inflammatory effects by suppressing inflammatory mediators through the NF-κB pathway, suggesting its potential to attenuate bronchial and pulmonary inflammation.
摘要：
急性肺损伤（ALI）是一种与急性呼吸衰竭相关的疾病，导致显著的发病率和死亡率。它涉及细胞变化，例如肺泡毛细血管膜的破坏，中性粒细胞过度迁移，和炎症介质的释放。Broncho-Vaxom®(BV)，一种冻干产品，含有来自呼吸道中常见的八种细菌的细胞膜成分，以减少病毒和细菌肺部感染的潜力而闻名。然而,BV对ALI的具体影响尚未明确。本研究探讨了BV在脂多糖（LPS）诱导的ALI小鼠模型中的预防作用及其潜在机制。气管内注射LPS前1小时口服BV(1mg/kg)灌胃,评价其对ALI模型的预防作用。BV的预施用显著减轻炎症参数,包括炎症介质的产生，巨噬细胞浸润，肺组织NF-κB激活,支气管肺泡灌洗液（BALF）中炎性细胞的增加。此外，BV(3μg/mL)预处理降低M1巨噬细胞标志物的表达,白细胞介素（IL-1β，IL-6),肿瘤坏死因子α,和环氧合酶-2，它们被LPS激活，小鼠肺泡巨噬细胞MH-S细胞和人巨噬细胞THP-1细胞。这些发现表明BV通过NF-κB途径抑制炎症介质而表现出抗炎作用。提示其减弱支气管和肺部炎症的潜力。
