PDF(Pubmed)
Abstract：
Tauroursodeoxycholic acid (TUDCA) increases the influx of primary bile acids into the gut. Results obtained on animal models suggested that Firmicutes and Proteobacteria phyla are more resistant to bile acids in rats. As part of a pilot study investigating the role of probiotics supplementation in elderly people with home enteral nutrition (HEN), a case of a 92-year-old woman with HEN is reported in the present study. She lives in a nursing home and suffers from Alzheimer\'s disease (AD); the patient had been prescribed TUDCA for lithiasis cholangitis. The aim of this case report is therefore to investigate whether long-term TUDCA administration may play a role in altering the patient\'s gut microbiota (GM) and the impact of an antibiotic therapy on the diversity of microbial species. Using next generation sequencing (NGS) analysis of the bacterial 16S ribosomal RNA (rRNA) gene a dominant shift toward Firmicutes and a remodeling in Proteobacteria abundance was observed in the woman\'s gut microbiota. Considering the patient\'s age, health status and type of diet, we would have expected to find a GM with a prevalence of Bacteroidetes phylum. This represents the first study investigating the possible TUDCA\'s effect on human GM.
摘要：
牛磺熊去氧胆酸(TUDCA)增加初级胆汁酸流入肠道。在动物模型上获得的结果表明，厚壁菌门和变形杆菌对大鼠胆汁酸的抗性更强。作为调查益生菌补充剂在家庭肠内营养（HEN）老年人中的作用的试点研究的一部分，本研究报告一例92岁女性患有HEN.她住在疗养院，患有阿尔茨海默氏病（AD）；该患者已接受TUDCA治疗结石性胆管炎。因此，本病例报告的目的是研究长期服用TUDCA是否可能在改变患者的肠道微生物群（GM）以及抗生素治疗对微生物多样性的影响方面发挥作用。使用细菌16S核糖体RNA（rRNA）基因的下一代测序（NGS）分析，在女性的肠道微生物群中观察到向Firmicutes的显性转变和变形杆菌丰度的重塑。考虑到病人的年龄,健康状况和饮食类型，我们本来希望找到一种流行有拟杆菌门的转基因。这是第一项研究TUDCA对人类GM可能的影响。
