PDF(Pubmed)
Abstract：
The oviduct provides an optimal environment for the final preparation, transport, and survival of gametes, the fertilization process, and early embryonic development. Most of the studies on reproduction are based on in vitro cell culture models because of the cell\'s accessibility. It creates opportunities to explore the complexity of directly linked processes between cells. Previous studies showed a significant expression of genes responsible for cell differentiation, maturation, and development during long-term porcine oviduct epithelial cells (POECs) in vitro culture. This study aimed at establishing the transcriptomic profile and comprehensive characteristics of porcine oviduct epithelial cell in vitro cultures, to compare changes in gene expression over time and deliver information about the expression pattern of genes highlighted in specific GO groups. The oviduct cells were collected after 7, 15, and 30 days of in vitro cultivation. The transcriptomic profile of gene expression was compared to the control group (cells collected after the first day). The expression of COL1A2 and LOX was enhanced, while FGFBP1, SERPINB2, and OVGP1 were downregulated at all selected intervals of cell culture in comparison to the 24-h control (p-value < 0.05). Adding new detailed information to the reproductive biology field about the diversified transcriptome profile in POECs may create new future possibilities in infertility treatments, including assisted reproductive technique (ART) programmes, and may be a valuable tool to investigate the potential role of oviduct cells in post-ovulation events.
摘要：
输卵管为最终准备提供了最佳环境，运输,和配子的存活，受精过程,和早期胚胎发育。由于细胞的可及性，大多数关于繁殖的研究都是基于体外细胞培养模型。它创造了探索细胞之间直接关联过程的复杂性的机会。以前的研究表明，负责细胞分化的基因显著表达，成熟,猪输卵管上皮细胞（POECs）长期体外培养过程中的发育。本研究旨在建立猪输卵管上皮细胞体外培养的转录组学图谱和综合特征,比较基因表达随时间的变化，并提供有关特定GO组中突出显示的基因表达模式的信息。在体外培养7、15和30天后收集输卵管细胞。将基因表达的转录组谱与对照组(第一天后收集的细胞)进行比较。COL1A2和LOX的表达增强，与24小时对照相比，FGFBP1，SERPINB2和OVGP1在细胞培养的所有选定间隔均下调（p值<0.05）。向生殖生物学领域添加有关POEC中多样化转录组概况的新详细信息，可能会在不孕症治疗中创造新的未来可能性，包括辅助生殖技术（ART）计划，并且可能是研究输卵管细胞在排卵后事件中的潜在作用的有价值的工具。
