PDF(Pubmed)
Abstract：
222 nm far-ultraviolet (F-UV) light has a bactericidal effect similar to deep-ultraviolet (D-UV) light of about a 260 nm wavelength. The cytotoxic effect of 222 nm F-UV has not been fully investigated. DLD-1 cells were cultured in a monolayer and irradiated with 222 nm F-UV or 254 nm D-UV. The cytotoxicity of the two different wavelengths of UV light was compared. Changes in cell morphology after F-UV irradiation were observed by time-lapse imaging. Differences in the staining images of DNA-binding agents Syto9 and propidium iodide (PI) and the amount of cyclobutane pyrimidine dimer (CPD) were examined after UV irradiation. F-UV was cytotoxic to the monolayer culture of DLD-1 cells in a radiant energy-dependent manner. When radiant energy was set to 30 mJ/cm2, F-UV and D-UV showed comparable cytotoxicity. DLD-1 cells began to expand immediately after 222 nm F-UV light irradiation, and many cells incorporated PI; in contrast, PI uptake was at a low level after D-UV irradiation. The amount of CPD, an indicator of DNA damage, was higher in cells irradiated with D-UV than in cells irradiated with F-UV. This study proved that D-UV induced apoptosis from DNA damage, whereas F-UV affected membrane integrity in monolayer cells.
摘要：
222nm远紫外(F-UV)光具有类似于波长约为260nm的深紫外(D-UV)光的杀菌作用。222nmF-UV的细胞毒性作用尚未得到充分研究。DLD-1细胞在单层中培养并用222nmF-UV或254nmD-UV照射。比较了两种不同波长的紫外光的细胞毒性。通过延时成像观察F-UV照射后细胞形态的变化。在UV照射后，检查了DNA结合剂Syto9和碘化丙啶（PI）的染色图像以及环丁烷嘧啶二聚体（CPD）的量的差异。F-UV以辐射能依赖性方式对DLD-1细胞的单层培养物具有细胞毒性。当辐射能设定为30mJ/cm2时,F-UV和D-UV显示出相当的细胞毒性。DLD-1细胞在222nmF-UV光照射后立即开始扩增,许多细胞掺入了PI；相比之下，在D-UV照射后,PI摄取处于低水平。CPD的数量，DNA损伤的指标,用D-UV照射的细胞比用F-UV照射的细胞更高。本研究证明了D-UV诱导的DNA损伤细胞凋亡,而F-UV影响单层细胞的膜完整性。
