PDF(Pubmed)
Abstract：
Selenium (Se) is an essential trace element for humans. Low concentrations of Se can promote plant growth and development. Enhancing grain yield and crop Se content is significant, as major food crops generally have low Se content. Studies have shown that Se biofortification can significantly increase Se content in plant tissues. In this study, the genetic transformation of wheat was conducted to evaluate the agronomic traits of non-transgenic control and transgenic wheat before and after Se application. Se content, speciation, and transfer coefficients in wheat grains were detected. Molecular docking simulations and transcriptome data were utilized to explore the effects of selenium-binding protein-A TaSBP-A on wheat growth and grain Se accumulation and transport. The results showed that TaSBP-A gene overexpression significantly increased plant height (by 18.50%), number of spikelets (by 11.74%), and number of grains in a spike (by 35.66%) in wheat. Under normal growth conditions, Se content in transgenic wheat grains did not change significantly, but after applying sodium selenite, Se content in transgenic wheat grains significantly increased. Analysis of Se speciation revealed that organic forms of selenomethionine (SeMet) and selenocysteine (SeCys) predominated in both W48 and transgenic wheat grains. Moreover, TaSBP-A significantly increased the transfer coefficients of Se from solution to roots and from flag leaves to grains. Additionally, it was found that with the increase in TaSBP-A gene overexpression levels in transgenic wheat, the transfer coefficient of Se from flag leaves to grains also increased.
摘要：
硒是人体必需的微量元素。低浓度的硒可以促进植物的生长发育。提高粮食产量和作物硒含量显著，作为主要粮食作物，硒含量普遍较低。研究表明，硒生物强化可以显着增加植物组织中的硒含量。在这项研究中,对小麦进行遗传转化，以评价施硒前后非转基因对照和转基因小麦的农艺性状。硒含量,物种形成,并检测了小麦籽粒的转移系数。利用分子对接模拟和转录组数据探索硒结合蛋白-ATaSBP-A对小麦生长和籽粒硒积累和运输的影响。结果表明,TaSBP-A基因过表达显著提高了株高(18.50%),小穗数量(减少11.74%)，小麦穗粒数(增加35.66%)。在正常生长条件下,转基因小麦籽粒中硒含量变化不显著,但是在使用亚硒酸钠后，转基因小麦籽粒中硒含量显著增加。硒形态的分析表明，硒代蛋氨酸（SeMet）和硒代半胱氨酸（SeCys）的有机形式在W48和转基因小麦籽粒中均占主导地位。此外，TaSBP-A显着增加了Se从溶液到根部以及从旗叶到谷物的转移系数。此外,研究发现,随着TaSBP-A基因在转基因小麦中过表达水平的增加,硒从旗叶向籽粒的转移系数也增加。
