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Abstract：
The aim of the present study was to analyze the association of the TLR2 (Toll-like receptor 2 gene) 2258G>A (rs5743708), TLR4 (Toll-like receptor 4 gene) 896A>G (rs4986790), and TLR4 1196C>T (rs4986791) polymorphisms with dental caries in Polish children. The participants, 261 15-year-old children, were divided into two groups: 82 cases (i.e., children with DMFT (Decayed, Missing, and Filled Teeth) index >5, having either moderate or high caries experience, assigned as the \"higher\" caries experience group) and 179 controls (i.e., children with DMFT ≤ 5, having either low or very low caries experience, assigned as the \"lower\" caries experience group). Genomic DNA was isolated from buccal swabs, and genotyping was determined by means of real-time PCR (polymerase chain reaction). There were no significant differences in the genotype or allele distributions in all tested SNPs (single nucleotide polymorphisms) between children with \"higher\" caries experience and those with \"lower\" caries experience. TLR4 haplotype frequencies did not differ significantly between cases and controls. In an additional analysis with another case definition applied (subjects with DMFT ≥ 1 were assigned as \"cases\", whereas children with DMFT = 0 were assigned as \"controls\"), no significant differences in the TLR2 and TLR4 genotype, allele frequencies, and TLR4 haplotype frequencies were found between the case and the control groups. The results of the present study broaden our knowledge on the potential genetic factors that might affect caries risk and suggest that TLR2 rs5743708 and TLR4 rs4986790 and rs4986791 SNPs are not associated with dental caries susceptibility in Polish children.
摘要：
本研究的目的是分析TLR2（Toll样受体2基因）2258G>A（rs5743708），TLR4(Toll样受体4基因)896A>G(rs4986790),TLR41196C>T(rs4986791)多态性与波兰儿童龋齿有关。参与者,261名15岁儿童，分为两组：82例(即患有DMFT的儿童（腐烂，失踪，和填充牙齿)指数>5，有中度或高度龋齿经历，分配为“更高”龋齿体验组)和179个对照(即DMFT≤5的儿童，有低或非常低的龋齿经验，分配为“较低”龋齿体验组)。基因组DNA是从口腔拭子中分离出来的,并通过实时PCR（聚合酶链反应）进行基因分型。在具有“较高”龋齿经历的儿童和具有“较低”龋齿经历的儿童之间，所有测试的SNP（单核苷酸多态性）的基因型或等位基因分布均无显着差异。TLR4单倍型频率在病例和对照之间没有显着差异。在应用另一个病例定义的额外分析中(DMFT≥1的受试者被分配为“病例”，而DMFT=0的孩子被分配为“对照”)，TLR2和TLR4基因型无显著差异,等位基因频率，在病例组和对照组之间发现TLR4单倍型频率。本研究的结果扩大了我们对可能影响龋齿风险的潜在遗传因素的认识，并表明TLR2rs5743708和TLR4rs4986790和rs4986791SNP与波兰儿童的龋齿易感性无关。
