PDF(Pubmed)
Abstract：
The acidic byproducts of bacteria in plaque around orthodontic brackets contribute to white spot lesion (WSL) formation. Nitric oxide (NO) has antibacterial properties, hindering biofilm formation and inhibiting the growth of oral microbes. Materials that mimic NO release could prevent oral bacteria-related pathologies. This study aims to integrate S-nitroso-acetylpenicillamine (SNAP), a promising NO donor, into orthodontic elastomeric ligatures, apply an additional polymer coating, and evaluate the NO-release kinetics and antimicrobial activity against Streptococus mutans. SNAP was added to clear elastomeric chains (8 loops, 23 mm long) at three concentrations (50, 75, 100 mg/mL, and a control). Chains were then coated, via electrospinning, with additional polymer (Elastollan®) to aid in extending the NO release. NO flux was measured daily for 30 days. Samples with 75 mg/mL SNAP + Elastollan® were tested against S. mutans for inhibition of biofilm formation on and around the chain. SNAP was successfully integrated into ligatures at each concentration. Only the 75 mg/mL SNAP chains maintained their elasticity. After polymer coating, samples exhibited a significant burst of NO on the first day, exceeding the machine\'s reading capacity, which gradually decreased over 29 days. Ligatures also inhibited S. mutans growth and biofilm formation. Future research will assess their mechanical properties and cytotoxicity. This study presents a novel strategy to address white spot lesion (WSL) formation and bacterial-related pathologies by utilizing nitric oxide-releasing materials. Manufactured chains with antimicrobial properties provide a promising solution for orthodontic challenges, showing significant potential for academic-industrial collaboration and commercial viability.
摘要：
正畸托槽周围牙菌斑中细菌的酸性副产物有助于白斑病变（WSL）的形成。一氧化氮（NO）具有抗菌性能，阻碍生物膜的形成和抑制口腔微生物的生长。模拟NO释放的材料可以预防口腔细菌相关的病变。本研究旨在整合S-亚硝基乙酰青霉胺（SNAP），一个有希望的没有捐赠者，进入正畸弹性结扎线，施加额外的聚合物涂层，并评估NO释放动力学和抗变形链球菌的抗菌活性。将SNAP添加到透明的弹性链中(8个环，23mm长）在三个浓度（50、75、100mg/mL，和控件)。然后涂覆链条，通过静电纺丝，添加额外的聚合物(Elastollan®)以帮助延长NO释放。每天测量NO通量,持续30天。测试具有75mg/mLSNAP+Elastollan®的样品针对变形链球菌对链上和链周围的生物膜形成的抑制。SNAP成功地整合到每个浓度的结扎物中。只有75mg/mLSNAP链保持其弹性。聚合物涂层后，样品在第一天表现出明显的NO爆发，超过机器的读取能力，在29天内逐渐下降。连接体还抑制变形链球菌生长和生物膜形成。未来的研究将评估其机械性能和细胞毒性。这项研究提出了一种通过利用一氧化氮释放材料来解决白斑病变（WSL）形成和细菌相关病理的新策略。具有抗菌性能的制造链提供了一个有前途的解决方案，正畸的挑战，显示出学术-工业合作和商业可行性的巨大潜力。
