PDF(Pubmed)
Abstract：
The lack of specific biological materials and biomarkers limits our knowledge of the mechanisms underlying intrauterine regulation of iron supply to the fetus. Determining the meconium content of proteins commonly used in the laboratory to assess the transport, storage, and distribution of iron in the body may elucidate their roles in fetal development. Ferritin, transferrin, haptoglobin, ceruloplasmin, lactoferrin, myeloperoxidase (MPO), neutrophil gelatinase-associated lipocalin (NGAL), and calprotectin were determined by ELISA in meconium samples obtained from 122 neonates. There were strong correlations between the meconium concentrations of haptoglobin, transferrin, and NGAL (p < 0.05). Meconium concentrations of ferritin were several-fold higher than the concentrations of the other proteins, with the exception of calprotectin whose concentration was approximately three-fold higher than that of ferritin. Meconium ceruloplasmin concentration significantly correlated with the concentrations of MPO, NGAL, lactoferrin, and calprotectin. Correlations between the meconium concentrations of haptoglobin, transferrin, and NGAL may reflect their collaborative involvement in the storage and transport of iron in the intrauterine environment in line with their recognized biological properties. High meconium concentrations of ferritin may provide information about the demand for iron and its utilization by the fetus. The associations between ceruloplasmin and neutrophil proteins may indicate the involvement of ceruloplasmin in the regulation of neutrophil activity in the intrauterine environment.
摘要：
缺乏特定的生物材料和生物标志物限制了我们对宫内调节胎儿铁供应的潜在机制的了解。确定实验室中常用蛋白质的胎粪含量，以评估运输，storage,铁在体内的分布可能阐明其在胎儿发育中的作用。铁蛋白,转铁蛋白,触珠蛋白，铜蓝蛋白,乳铁蛋白,髓过氧化物酶(MPO),中性粒细胞明胶酶相关脂质运载蛋白（NGAL），通过ELISA在122例新生儿的胎粪样品中测定钙卫蛋白。结合珠蛋白的胎粪浓度之间存在很强的相关性，转铁蛋白,和NGAL(p<0.05)。铁蛋白的胎粪浓度比其他蛋白质的浓度高几倍，除了钙卫蛋白的浓度比铁蛋白高约三倍。胎粪铜蓝蛋白浓度与MPO浓度显著相关,NGAL,乳铁蛋白,还有钙卫蛋白.结合珠蛋白的胎粪浓度之间的相关性，转铁蛋白,和NGAL可能反映了它们在宫内环境中的铁的储存和运输中的协作参与，符合其公认的生物学特性。高胎粪浓度的铁蛋白可能提供有关胎儿对铁的需求及其利用的信息。铜蓝蛋白和中性粒细胞蛋白之间的关联可能表明铜蓝蛋白参与了子宫内环境中中性粒细胞活性的调节。
