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Abstract：
Solid tumors as well as leukemias and lymphomas show striking changes in nuclear structure including nuclear size and shape, the number and size of nucleoli, and chromatin texture. These alterations have been used in cancer diagnosis and might be related to the altered functional properties of cancer cells. The nuclear matrix (NM) represents the structural composition of the nucleus and consists of nuclear lamins and pore complexes, an internal ribonucleic protein network, and residual nucleoli. In the nuclear microenvironment, the NM is associated with multi-protein complexes, such as basal transcription factors, signaling proteins, histone-modifying factors, and chromatin remodeling machinery directly or indirectly through scaffolding proteins. Therefore, alterations in the composition of NM could result in altered DNA topology and changes in the interaction of various genes, which could then participate in a cascade of the cancer process. Using an androgen-sensitive prostate cancer cell line, LNCaP, and its androgen-independent derivative, LN96, conventional 2D-proteomic analysis of the NM proteins revealed that purine-rich element binding protein alpha (PURα) was detected in the NM proteins and differentially expressed between the cell lines. In this article, we will review the potential role of the molecule in prostate cancer.
摘要：
实体肿瘤以及白血病和淋巴瘤显示出显著的核结构变化，包括核大小和形状，核仁的数量和大小,和染色质纹理。这些改变已用于癌症诊断，可能与癌细胞的功能特性改变有关。核基质（NM）代表核的结构组成，由核层和孔复合物组成，内部的核糖核酸蛋白质网络，和残留的核仁.在核微环境中，NM与多蛋白复合物有关，如基础转录因子，信号蛋白，组蛋白修饰因子，和染色质重塑机制直接或间接通过支架蛋白。因此,NM组成的改变可能导致DNA拓扑结构的改变和各种基因相互作用的变化，然后可以参与癌症过程的级联。使用雄激素敏感的前列腺癌细胞系，LNCaP,和它的雄激素非依赖性衍生物，LN96，NM蛋白的常规2D蛋白质组学分析显示，在NM蛋白中检测到富含嘌呤的元件结合蛋白α（PURα），并在细胞系之间差异表达。在这篇文章中,我们将回顾该分子在前列腺癌中的潜在作用。
