PDF(Pubmed)
Abstract：
Though the microbiome\'s impact on immune system homeostasis is well documented, the effect of circulating T cells on the gut microbiome remains unexamined. We analyzed data from 50 healthy volunteers in a pilot trial of aspirin, using immunophenotyping and 16S rRNA sequencing to evaluate the effect of baseline T cells on microbiome changes over 6 weeks. We employed an unsupervised sparse canonical correlation analysis (sCCA) and used multivariable linear regression models to evaluate the association between selected T cell subsets and selected bacterial genera after adjusting for covariates. In the cross-sectional analysis, percentages of naïve CD4+ T cells were positively associated with a relative abundance of Intestinimonas, and the percentage of activated CD8+ T cells was inversely associated with Cellulosibacter. In the longitudinal analysis, the baseline percentages of naïve CD4+ T cells and activated CD4+ T cells were inversely associated with a 6-week change in the relative abundance of Clostridium_XlVb and Anaerovorax, respectively. The baseline percentage of terminal effector CD4+ T cells was positively associated with the change in Flavonifractor. Notably, the microbiome taxa associated with T cell subsets exclusively belonged to the Bacillota phylum. These findings can guide future experimental studies focusing on the role of T cells in impacting gut microbiome homeostasis.
摘要：
虽然微生物组对免疫系统稳态的影响是有据可查的，循环T细胞对肠道微生物组的影响仍未研究。我们分析了50名健康志愿者在阿司匹林试验中的数据，使用免疫表型和16SrRNA测序来评估基线T细胞对6周内微生物组变化的影响。我们采用了无监督的稀疏典型相关分析（sCCA），并使用多变量线性回归模型在调整协变量后评估了选定的T细胞亚群和选定的细菌属之间的关联。在横截面分析中，初始CD4+T细胞的百分比与肠单胞菌的相对丰度呈正相关，激活的CD8+T细胞的百分比与Cellulosibacter呈负相关。在纵向分析中，初始CD4+T细胞和活化CD4+T细胞的基线百分比与梭菌XlVb和厌氧菌相对丰度的6周变化呈负相关，分别。终末效应CD4+T细胞的基线百分比与Flavonifractor的变化呈正相关。值得注意的是,与T细胞亚群相关的微生物组分类群完全属于芽孢杆菌门。这些发现可以指导未来的实验研究，重点关注T细胞在影响肠道微生物组稳态中的作用。
